Product nameAnti-DENN antibody
See all DENN primary antibodies
DescriptionRabbit polyclonal to DENN
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Synthetic peptide within Mouse DENN (internal sequence). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: Q80U28
- WB: Neuro-2a and RAW 264.7 whole cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 175 kDa.|
FunctionPlays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Isoform 5 shows increased cell proliferation and isoform 2 shows decreased. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis); isoform 1 is susceptible to inducing apoptosis, isoform 5 is resistant and isoform 3 and isoform 4 have no effect.
Tissue specificityHighly expressed in fetal brain and kidney; adult testis, ovary, brain and heart. Isoform 5 is constitutively expressed in all tissues. Isoform 7 is expressed in fetal liver and in several cancer cell lines.
Sequence similaritiesBelongs to the MADD family.
Contains 1 dDENN domain.
Contains 1 death domain.
Contains 1 DENN domain.
Contains 1 uDENN domain.
- Information by UniProt
- Differentially expressed in normal and neoplastic cells antibody
- FLJ35600 antibody
- IG20 antibody
All lanes : Anti-DENN antibody (ab229572) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 175 kDa
5% SDS-PAGE gel.
ab229572 has not yet been referenced specifically in any publications.