Key features and details
- Rabbit polyclonal to DENN
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-DENN antibody
See all DENN primary antibodies
DescriptionRabbit polyclonal to DENN
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Synthetic peptide within Mouse DENN (internal sequence). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: Q80U28
- WB: Neuro-2a and RAW 264.7 whole cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 175 kDa.|
FunctionPlays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Isoform 5 shows increased cell proliferation and isoform 2 shows decreased. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis); isoform 1 is susceptible to inducing apoptosis, isoform 5 is resistant and isoform 3 and isoform 4 have no effect.
Tissue specificityHighly expressed in fetal brain and kidney; adult testis, ovary, brain and heart. Isoform 5 is constitutively expressed in all tissues. Isoform 7 is expressed in fetal liver and in several cancer cell lines.
Sequence similaritiesBelongs to the MADD family.
Contains 1 dDENN domain.
Contains 1 death domain.
Contains 1 DENN domain.
Contains 1 uDENN domain.
- Information by UniProt
- Differentially expressed in normal and neoplastic cells antibody
- FLJ35600 antibody
- IG20 antibody
All lanes : Anti-DENN antibody (ab229572) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 175 kDa
5% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229572 has not yet been referenced specifically in any publications.