Overview

  • Product name

  • Description

    Rabbit polyclonal to DENN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse DENN (internal sequence). The exact sequence is proprietary. (Carrier-protein conjugated).
    Database link: Q80U28

  • Positive control

    • WB: Neuro-2a and RAW 264.7 whole cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab229572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 175 kDa.

Target

  • Function

    Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Isoform 5 shows increased cell proliferation and isoform 2 shows decreased. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis); isoform 1 is susceptible to inducing apoptosis, isoform 5 is resistant and isoform 3 and isoform 4 have no effect.
  • Tissue specificity

    Highly expressed in fetal brain and kidney; adult testis, ovary, brain and heart. Isoform 5 is constitutively expressed in all tissues. Isoform 7 is expressed in fetal liver and in several cancer cell lines.
  • Sequence similarities

    Belongs to the MADD family.
    Contains 1 dDENN domain.
    Contains 1 death domain.
    Contains 1 DENN domain.
    Contains 1 uDENN domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Differentially expressed in normal and neoplastic cells antibody
    • FLJ35600 antibody
    • IG20 antibody
    • Insulinoma glucagonoma clone 20 antibody
    • KIAA0358 antibody
    • MADD antibody
    • MADD_HUMAN antibody
    • MAP kinase-activating death domain protein antibody
    • Rab3 GDP/GTP exchange factor antibody
    • RAB3GEP antibody
    see all

Images

  • All lanes : Anti-DENN antibody (ab229572) at 1/1000 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
    Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 175 kDa



    5% SDS-PAGE gel.

References

ab229572 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229572.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up