Overview

  • Product name

    Anti-DENN antibody [EPR4919]
    See all DENN primary antibodies
  • Description

    Rabbit monoclonal [EPR4919] to DENN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human DENN (C terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa cell lysate, 293T cell lysate, K562 cell lysate, NIH 3T3 cell lysate, Human breast tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 220 kDa (predicted molecular weight: 183 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Isoform 5 shows increased cell proliferation and isoform 2 shows decreased. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis); isoform 1 is susceptible to inducing apoptosis, isoform 5 is resistant and isoform 3 and isoform 4 have no effect.
    • Tissue specificity

      Highly expressed in fetal brain and kidney; adult testis, ovary, brain and heart. Isoform 5 is constitutively expressed in all tissues. Isoform 7 is expressed in fetal liver and in several cancer cell lines.
    • Sequence similarities

      Belongs to the MADD family.
      Contains 1 dDENN domain.
      Contains 1 death domain.
      Contains 1 DENN domain.
      Contains 1 uDENN domain.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Differentially expressed in normal and neoplastic cells antibody
      • FLJ35600 antibody
      • IG20 antibody
      • Insulinoma glucagonoma clone 20 antibody
      • KIAA0358 antibody
      • MADD antibody
      • MADD_HUMAN antibody
      • MAP kinase-activating death domain protein antibody
      • Rab3 GDP/GTP exchange factor antibody
      • RAB3GEP antibody
      see all

    Images

    • All lanes : Anti-DENN antibody [EPR4919] (ab134117) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 183 kDa
      Observed band size: 220 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemical analysis of paraffin embedded Human breast tissue labelling DENN with ab134117 at 1/100.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab134117 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134117.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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