Anti-DENND4A antibody (ab117758)
Key features and details
- Rabbit polyclonal to DENND4A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DENND4A antibody -
Description
Rabbit polyclonal to DENND4A -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Orangutan -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 1525-1575 of Human DENND4A (NP_005839.3).
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Positive control
- Jurkat, 293T and HeLa whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 209 kDa.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 209 kDa. |
Target
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Function
Binds to ISRE-like element (interferon-stimulated response element) of MYC P2 promoter. -
Tissue specificity
Expressed ubiquitously. Highest expression in bone marrow, medium in peripheral blood lymphocytes and lowest in spleen. In brain, breast, and prostate, higher expression was seen in normal cells than in tumor cells. Expression is regulated in a growth- and cell cycle-dependent manner. -
Sequence similarities
Contains 1 dDENN domain.
Contains 1 DENN domain.
Contains 1 MABP domain.
Contains 2 PPR (pentatricopeptide) repeats.
Contains 1 uDENN domain. -
Developmental stage
Highly expressed in fetal liver. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10260 Human
- Omim: 600382 Human
- SwissProt: Q7Z401 Human
- Unigene: 654567 Human
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Alternative names
- C-myc promoter-binding protein antibody
- DENN domain-containing protein 4A antibody
- DENN/MADD domain containing 4A antibody
see all
Images
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All lanes : Anti-DENND4A antibody (ab117758) at 0.4 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 209 kDa
Exposure time: 30 seconds
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab117758 has not yet been referenced specifically in any publications.