Denosumab ELISA Kit (ab237649)
Key features and details
- Sensitivity: 3 ng/ml
- Range: 3 ng/ml - 300 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Denosumab ELISA Kit
See all Denosumab kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 15% Inter-assay Sample n Mean SD CV% Overall < 15% -
Sample type
Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3 ng/ml -
Range
3 ng/ml - 300 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 85% - 115% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Note: Denosumab ELISA kit ab237649 is intended for Research Use Only, not for use in diagnostic procedures.
This kit is a sandwich based ELISA kit. The color developed is proportional to the amount of Denosumab in the sample or standard. Results of samples can be determined directly using the standard curve.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called E4380 BioSim™ Denosumab (Human) ELISA Kit. E4380-100 is the same size as the 96 test size of ab237649.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests Micro ELISA Plate 1 unit Denosumab Standard S1 1 x 1ml Denosumab Standard S2 1 x 1ml Denosumab Standard S3 1 x 1ml Denosumab Standard S4 1 x 1ml Denosumab Standard S5 1 x 1ml Denosumab Standard S6 1 x 1ml Denosumab Standard S7 1 x 1ml Assay Buffer 1 x 50ml HRP-conjugate Probe 1 x 12ml TMB substrate 1 x 12ml Stop Solution 1 x 12ml Wash buffer (20X) 1 x 50ml Plate sealers 2 units -
Relevance
Denosumab is a human monoclonal antibody that binds to RANKL, a transmembrane or soluble protein essential for the formation, function, and survival of osteoclasts, the cells responsible for bone resorption. Prolia prevents RANKL from activating its receptor, RANK, on the surface of osteoclasts and their precursors. Prevention of the RANKL/RANK interaction inhibits osteoclast formation, function, and survival, thereby decreasing bone resorption and increasing bone mass and strength in both cortical and trabecular bone. -
Alternative names
- Prolia
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab237649 has not yet been referenced specifically in any publications.