Key features and details
- Sensitivity: 3 ng/ml
- Range: 3 ng/ml - 300 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameDenosumab ELISA Kit
See all Denosumab kits
Intra-assay Sample n Mean SD CV% Overall < 15% Inter-assay Sample n Mean SD CV% Overall < 15%
Sample typeSerum, Plasma
Assay typeSandwich (quantitative)
Range3 ng/ml - 300 ng/ml
Sample specific recovery Sample type Average % Range Serum 85% - 115%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Note: Denosumab ELISA kit ab237649 is intended for Research Use Only, not for use in diagnostic procedures.
This kit is a sandwich based ELISA kit. The color developed is proportional to the amount of Denosumab in the sample or standard. Results of samples can be determined directly using the standard curve.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 96 tests Micro ELISA Plate 1 unit Denosumab Standard S1 1 x 1ml Denosumab Standard S2 1 x 1ml Denosumab Standard S3 1 x 1ml Denosumab Standard S4 1 x 1ml Denosumab Standard S5 1 x 1ml Denosumab Standard S6 1 x 1ml Denosumab Standard S7 1 x 1ml Denosumab Standard S8 1 x 1ml Assay Buffer 1 x 50ml HRP-conjugate Probe 1 x 12ml TMB substrate 1 x 12ml Stop Solution 1 x 12ml Wash buffer (20X) 1 x 50ml Plate sealers 2 units
RelevanceDenosumab is a human monoclonal antibody that binds to RANKL, a transmembrane or soluble protein essential for the formation, function, and survival of osteoclasts, the cells responsible for bone resorption. Prolia prevents RANKL from activating its receptor, RANK, on the surface of osteoclasts and their precursors. Prevention of the RANKL/RANK interaction inhibits osteoclast formation, function, and survival, thereby decreasing bone resorption and increasing bone mass and strength in both cortical and trabecular bone.
ab237649 has not yet been referenced specifically in any publications.