Overview

  • Product name

    Denosumab ELISA Kit (Prolia®)
    See all Denosumab kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 15%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 15%
  • Sample type

    Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    3 ng/ml
  • Range

    3 ng/ml - 300 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 85% - 115%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Denosumab ELISA Kit (Prolia®) (ab237649) has been developed for specific quantification of Denosumab concentration in human serum or plasma with high sensitivity and reproducibility. The density of color is proportional to the amount of Denosumab captured from the samples and can be quantified when compared with standard curve.


    Denosumab (Prolia®) is a human monoclonal antibody that binds to RANKL, a transmembrane or soluble protein essential for the formation, function, and survival of osteoclasts, the cells responsible for bone resorption. Prolia prevents RANKL from activating its receptor, RANK, on the surface of osteoclasts and their precursors. Prevention of the RANKL/RANK interaction inhibits osteoclast formation, function, and survival, thereby decreasing bone resorption and increasing bone mass and strength in both cortical and trabecular bone.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 96 tests
    Micro ELISA Plate 1 unit
    Denosumab Standard S1 1 x 1ml
    Denosumab Standard S2 1 x 1ml
    Denosumab Standard S3 1 x 1ml
    Denosumab Standard S4 1 x 1ml
    Denosumab Standard S5 1 x 1ml
    Denosumab Standard S6 1 x 1ml
    Denosumab Standard S7 1 x 1ml
    Denosumab Standard S8 1 x 1ml
    Assay Buffer 1 x 50ml
    HRP-conjugate Probe 1 x 12ml
    TMB substrate 1 x 12ml
    Stop Solution 1 x 12ml
    Wash buffer (20X) 1 x 50ml
    Plate sealers 2 units
  • Relevance

    Denosumab (Prolia®) is a human monoclonal antibody that binds to RANKL, a transmembrane or soluble protein essential for the formation, function, and survival of osteoclasts, the cells responsible for bone resorption. Prolia prevents RANKL from activating its receptor, RANK, on the surface of osteoclasts and their precursors. Prevention of the RANKL/RANK interaction inhibits osteoclast formation, function, and survival, thereby decreasing bone resorption and increasing bone mass and strength in both cortical and trabecular bone.
  • Alternative names

    • Prolia

Applications

Our Abpromise guarantee covers the use of ab237649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.

Protocols

References

ab237649 has not yet been referenced specifically in any publications.

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