Overview

  • Product name

    anti-Denosumab ELISA Kit (Prolia®)
    See all Denosumab kits
  • Detection method

    Colorimetric
  • Sample type

    Serum, Plasma
  • Assay type

    Sandwich (qualitative)
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    anti-Denosumab ELISA Kit (Prolia®) (ab237663) is a highly specific and sensitive kit designed for the in vitro determination of the antibody against Denosumab in biological matrices such as human serum and plasma.


    Denosumab (Prolia®) is a human monoclonal antibody that binds to RANKL, a transmembrane or soluble protein essential for the formation, function, and survival of osteoclasts, the cells responsible for bone resorption. Prolia prevents RANKL from activating its receptor, RANK, on the surface of osteoclasts and their precursors. Prevention of the RANKL/RANK interaction inhibits osteoclast formation, function, and survival, thereby decreasing bone resorption and increasing bone mass and strength in both cortical and trabecular bone. However, some patients develop unwanted immunogenicity, which leads to production of anti-drug-antibodies (ADAs) inactivating the therapeutic effects of the treatment and, in rare cases, inducing adverse effects.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 96 tests
    Micro ELISA Plate 1 unit
    Positive Control 1 x 0.25ml
    Negative Control 1 x 0.5ml
    Assay Buffer 1 x 12ml
    Peroxidase Conjugate 1 x 12ml
    TMB substrate 1 x 12ml
    Stop Solution 1 x 12ml
    Wash buffer (20X) 1 x 50ml
    Plate sealers 2 units
  • Relevance

    Denosumab (Prolia®) is a human monoclonal antibody that binds to RANKL, a transmembrane or soluble protein essential for the formation, function, and survival of osteoclasts, the cells responsible for bone resorption. Prolia prevents RANKL from activating its receptor, RANK, on the surface of osteoclasts and their precursors. Prevention of the RANKL/RANK interaction inhibits osteoclast formation, function, and survival, thereby decreasing bone resorption and increasing bone mass and strength in both cortical and trabecular bone.
  • Alternative names

    • Prolia

Applications

Our Abpromise guarantee covers the use of ab237663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Protocols

References

ab237663 has not yet been referenced specifically in any publications.

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