The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/1. Predicted molecular weight: 79 kDa. Preliminary experiments in Human Testis lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml).
Part of the CASK/TRB1/TSPYL2 transcriptional complex which modulates gene expression in response to neuronal synaptic activity, probably by facilitating nucleosome assembly. May inhibit cell proliferation by inducing p53-dependent CDKN1A expression.
Ubiquitously expressed, with highest levels in brain, testis and heart, and lowest levels in liver and pancreas.
Belongs to the nucleosome assembly protein (NAP) family.
Phosphorylation at Ser-20 and/or Thr-340 impairs function on cell proliferation.
Nucleus. Cytoplasm. Enriched in transcriptionally active regions of chromatin in neurons.