Storage instructionsShipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Purification notesab140236 is purified from cell culture supernatant by protein A affinity chromatography to a purity of > 95% (by SDS-PAGE).
Our Abpromise guarantee covers the use of ab140236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionTyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, PDGFRB, MET, RET (variant MEN2A), KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Involved in vascular development. Regulator of macrophage adhesion and spreading. Positively affects cell-matrix adhesion. Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation. Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling.
Tissue specificityExpressed in the promyelocytic cell line HL60, the granulocyte-macrophage colony-stimulating factor-dependent leukemic cell line F-36P, and the interleukin-3 and erythropoietin-dependent leukemic cell line F-36E. Expressed predominantly in epithelial cells and lymphocytes. Enhanced expression at high cell density.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily.
Contains 9 fibronectin type-III domains.
Contains 1 tyrosine-protein phosphatase domain.
modificationsN- and O-glycosylated.
Cellular localizationCell membrane. Cell projection > ruffle membrane. Cell junction. After T cell stimulation, it is temporarily excluded from immunological synapses.
- Information by UniProt
- CD 148 antibody
- CD148 antibody
- CD148 antigen antibody
This product has been referenced in:
- Clayton KL et al. T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases. J Immunol 192:782-91 (2014). Human . Read more (PubMed: 24337741) »