Key features and details
- Rabbit polyclonal to DEPDC1B
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DEPDC1B antibody
DescriptionRabbit polyclonal to DEPDC1B
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cynomolgus monkey
Recombinant fragment corresponding to Human DEPDC1B aa 343-529.
MPPLCDGFGTRTLMVQTFSRCILCSKDEVDLDELLAARLVTFLMDNYQEI LKVPLALQTSIEERVAHLRRVQIKYPGADMDITLSAPSFCRQISPEEFEY QRSYGSQEPLAALLEEVITDAKLSNKEKKKKLKQFQKSYPEVYQERFPTP ESAALLFPEKPKPKPQLLMWALKKPFQPFQRTRSFRM
Database link: Q8WUY9-1
- IHC-P: Human testis and liver cancer tissues. ICC/IF: HepG2 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
RelevanceGTPase activator activity. biological process: intracellular signaling cascade
- BRCC3 antibody
- Breast cancer cell 3 antibody
- DEP domain containing 1B antibody
HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for DEPDC1B (green) using ab237542 at 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
Paraffin-embedded human testis tissue stained for DEPDC1B using ab237542 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human liver cancer tissue stained for DEPDC1B using ab237542 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237542 has not yet been referenced specifically in any publications.