Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20497-23] to DEPDC5
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse
Product nameAnti-DEPDC5 antibody [EPR20497-23]
See all DEPDC5 primary antibodies
DescriptionRabbit monoclonal [EPR20497-23] to DEPDC5
Tested applicationsSuitable for: WBmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse
Synthetic peptide within Human DEPDC5 aa 1500-1600. The exact sequence is proprietary.
Database link: O75140
- WB: Wild-type mouse E14 brain lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab213181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 181 kDa (predicted molecular weight: 181 kDa).|
Sequence similaritiesBelongs to the IML1 family.
Contains 1 DEP domain.
- Information by UniProt
- DEP domain containing 5 antibody
- DEP domain-containing protein 5 antibody
- DEP.5 antibody
All lanes : Anti-DEPDC5 antibody [EPR20497-23] (ab213181) at 1/1000 dilution
Lane 1 : Wild-type mouse E14 brain tissue lysate
Lane 2 : DEPDC5 knockout mouse E14 brain tissue lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 181 kDa
Observed band size: 181 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The wild-type and DEPDC5 knockout mouse E14 brain tissue lysates were kindly provided by an anonymous collaborator.
ab213181 was shown to specifically react with DEPDC5 in wild-type mouse E14 brain tissue as signal was lost in DEPDC5 knockout tissue. Wild-type and DEPDC5 knockout samples were subjected to SDS-PAGE. ab213181 and ab129002 (Rabbit anti-Vinculin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/5000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100, 000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
Exposure time: 26 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab213181 has not yet been referenced specifically in any publications.