Recombinant Anti-DEPDC5 antibody [EPR20497-23] (ab213181)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20497-23] to DEPDC5
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-DEPDC5 antibody [EPR20497-23]
See all DEPDC5 primary antibodies -
Description
Rabbit monoclonal [EPR20497-23] to DEPDC5 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Does not react with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type mouse E14 brain lysate, Wild-type A549 cell lysate, Mouse Brain cell lysate, Human Brain cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20497-23 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab213181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000. Detects a band of approximately 181 kDa (predicted molecular weight: 181 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 181 kDa (predicted molecular weight: 181 kDa). |
Target
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Sequence similarities
Belongs to the IML1 family.
Contains 1 DEP domain. - Information by UniProt
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Database links
- Entrez Gene: 9681 Human
- Entrez Gene: 277854 Mouse
- Omim: 614191 Human
- SwissProt: O75140 Human
- SwissProt: P61460 Mouse
- Unigene: 435022 Human
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Alternative names
- DEP domain containing 5 antibody
- DEP domain-containing protein 5 antibody
- DEP.5 antibody
see all
Images
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All lanes : Anti-DEPDC5 antibody [EPR20497-23] (ab213181) at 1/1000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : DEPDC5 knockout A549 cell lysate
Lane 3 : Human brain tissue lysate
Lane 4 : Mouse brain tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 181 kDa
Observed band size: 181 kDaFalse colour image of Western blot: Anti-DEPDC5 antibody [EPR20497-23] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab213181 was shown to bind specifically to DEPDC5. A band was observed at 181 kDa in wild-type A549 cell lysates with no signal observed at this size in DEPDC5 knockout cell line ab266906 (knockout cell lysate ab258394). To generate this image, wild-type and DEPDC5 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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All lanes : Anti-DEPDC5 antibody [EPR20497-23] (ab213181) at 1/1000 dilution
Lane 1 : Wild-type mouse E14 brain tissue lysate
Lane 2 : DEPDC5 knockout mouse E14 brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 181 kDa
Observed band size: 181 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The wild-type and DEPDC5 knockout mouse E14 brain tissue lysates were kindly provided by an anonymous collaborator.
ab213181 was shown to specifically react with DEPDC5 in wild-type mouse E14 brain tissue as signal was lost in DEPDC5 knockout tissue. Wild-type and DEPDC5 knockout samples were subjected to SDS-PAGE. ab213181 and ab129002 (Rabbit anti-Vinculin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/5000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100, 000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
Exposure time: 26 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab213181 has been referenced in 1 publication.
- Valenstein ML et al. Structure of the nutrient-sensing hub GATOR2. Nature 607:610-616 (2022). PubMed: 35831510