Recombinant Anti-DEPDC5 antibody [EPR20497-23] - BSA and Azide free (ab272399)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20497-23] to DEPDC5 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
-
Product name
Anti-DEPDC5 antibody [EPR20497-23] - BSA and Azide free
See all DEPDC5 primary antibodies -
Description
Rabbit monoclonal [EPR20497-23] to DEPDC5 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Does not react with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Wild-type mouse E14 brain lysate. A549 cell lysate. Human Brain cell lysate
-
General notes
ab272399 is the carrier-free version of ab213181.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20497-23 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Detects a band of approximately 181 kDa (predicted molecular weight: 181 kDa).
|
Notes |
---|
WB
Use at an assay dependent concentration. Detects a band of approximately 181 kDa (predicted molecular weight: 181 kDa). |
Target
-
Sequence similarities
Belongs to the IML1 family.
Contains 1 DEP domain. - Information by UniProt
-
Database links
- Entrez Gene: 9681 Human
- Entrez Gene: 277854 Mouse
- Omim: 614191 Human
- SwissProt: O75140 Human
- SwissProt: P61460 Mouse
- Unigene: 435022 Human
-
Alternative names
- DEP domain containing 5 antibody
- DEP domain-containing protein 5 antibody
- DEP.5 antibody
see all
Images
-
All lanes : Anti-DEPDC5 antibody [EPR20497-23] (ab213181) at 1/1000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : DEPDC5 knockout A549 cell lysate
Lane 3 : Human brain tissue lysate
Lane 4 : Mouse brain tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 181 kDa
Observed band size: 181 kDaFalse colour image of Western blot: Anti-DEPDC5 antibody [EPR20497-23] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab213181 was shown to bind specifically to DEPDC5. A band was observed at 181 kDa in wild-type A549 cell lysates with no signal observed at this size in DEPDC5 knockout cell line ab266906 (knockout cell lysate ab258394). To generate this image, wild-type and DEPDC5 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
-
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The wild-type and DEPDC5 knockout mouse E14 brain tissue lysates were kindly provided by an anonymous collaborator.
ab213181 was shown to specifically react with DEPDC5 in wild-type mouse E14 brain tissue as signal was lost in DEPDC5 knockout tissue. Wild-type and DEPDC5 knockout samples were subjected to SDS-PAGE. ab213181 and ab129002 (Rabbit anti-Vinculin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/5000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100, 000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
Exposure time: 26 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab213181).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab272399 has not yet been referenced specifically in any publications.