Key features and details
- Sample type: Cell Lysate, Tissue Homogenate, Urine
Product nameDeproteinizing Sample Preparation Kit
See all Deproteinizing sample kits
Sample typeUrine, Cell Lysate, Tissue Homogenate
Assay durationMultiple steps standard assay
The Deproteinizing Sample Preparation Kit (ab284939) (K808) utilizes a PCA (perchloric acid) precipitation method, providing a unique tool to prepare samples for the analysis of a variety of small molecules. Using the kit, proteins are precipitated, excess PCA is removed, samples are neutralized. Samples prepared using this kit can be directly used in a wide variety of bioassays.
The method is easy, convenient, and can be used for the preparation of a large number of samples.
Different amounts of PCA were used to deproteinize 400 µL of serum following the kit protocol. Protein concentration remaining in solution OD280 (♦) and sample pH (•) was measured. Samples (neat serum ; serum filtered through a 10kD MW cutoff ; and PCA treated ) were diluted 20 times for convenience of measuring OD at 280 nm. A separate aliquot of the same PCA treated samples was mixed 1:1 with a pH 7.75 assay buffer and the pH of the mixture determined. The figure shows that sample/PCA ratio errors up to 20% can be tolerated with the resulting assay pH holding within approximately 0.5 pH units of the target assay pH. Using a 10 kDa MW cutoff filter removes approximately 98% of protein and PCA precipitation at a PCA/sample ratio of 1:4 removes approximately 95% of protein present.
ab284939 has not yet been referenced specifically in any publications.