Anti-Deptor antibody (ab103298)
Key features and details
- Rabbit polyclonal to Deptor
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Deptor antibody
See all Deptor primary antibodies -
Description
Rabbit polyclonal to Deptor -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Full length protein, corresponding to amino acids 1-409 of Human Deptor (AAH12040.1).
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Positive control
- Mouse liver tissue lysate; Deptor transfected 293T cell line
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab103298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 46 kDa. |
Target
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Function
Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes. -
Sequence similarities
Contains 2 DEP domains.
Contains 1 PDZ (DHR) domain. -
Post-translational
modificationsPhosphorylated. Phosphorylation weakens interaction with MTOR within mTORC1 and mTORC2. - Information by UniProt
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Database links
- Entrez Gene: 64798 Human
- Entrez Gene: 97998 Mouse
- Omim: 612974 Human
- SwissProt: Q8TB45 Human
- SwissProt: Q570Y9 Mouse
- Unigene: 112981 Human
- Unigene: 295397 Mouse
- Unigene: 393497 Mouse
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Alternative names
- DEP domain containing 6 antibody
- DEP domain-containing mTOR-interacting protein antibody
- DEP domain-containing protein 6 antibody
see all
Images
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Western blot - Anti-Deptor antibody (ab103298)Anti-Deptor antibody (ab103298) at 1/500 dilution + Mouse liver tissue lysate at 50 µg
Predicted band size: 46 kDa -
Western blot - Anti-Deptor antibody (ab103298)All lanes : Anti-Deptor antibody (ab103298) at 1/500 dilution
Lane 1 : Deptor transfected 293T cell line lysate
Lane 2 : non transfected 293T cell line lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 46 kDa
Datasheets and documents
References (0)
ab103298 has not yet been referenced specifically in any publications.
