Anti-Deptor antibody (ab244395)
Key features and details
- Rabbit polyclonal to Deptor
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Deptor antibody
See all Deptor primary antibodies -
Description
Rabbit polyclonal to Deptor -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment corresponding to Human Deptor aa 200-350.
Database link: Q8TB45 -
Positive control
- ICC/IF: MCF7 cells. WB: Deptor overexpression HEK-293T cell lysate. IHC-P: Human skeletal muscle, fallopian tube and small intestine tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab244395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes. -
Sequence similarities
Contains 2 DEP domains.
Contains 1 PDZ (DHR) domain. -
Post-translational
modificationsPhosphorylated. Phosphorylation weakens interaction with MTOR within mTORC1 and mTORC2. - Information by UniProt
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Database links
- Entrez Gene: 64798 Human
- Entrez Gene: 97998 Mouse
- Omim: 612974 Human
- SwissProt: Q8TB45 Human
- SwissProt: Q570Y9 Mouse
- Unigene: 112981 Human
- Unigene: 295397 Mouse
- Unigene: 393497 Mouse
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Alternative names
- DEP domain containing 6 antibody
- DEP domain-containing mTOR-interacting protein antibody
- DEP domain-containing protein 6 antibody
see all
Images
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PFA-fixed, Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells stained for Deptor (green) using ab244395 at 4 µg/ml in ICC/IF.
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Paraffin-embedded human small intestine tissue stained for Deptor using ab244395 at 1/50 dilution in immunohistochemical analysis.
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Paraffin-embedded human fallopian tube tissue stained for Deptor using ab244395 at 1/50 dilution in immunohistochemical analysis.
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Paraffin-embedded human skeletal muscle tissue stained for Deptor using ab244395 at 1/50 dilution in immunohistochemical analysis.
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All lanes : Anti-Deptor antibody (ab244395) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : Deptor overexpression HEK-293T cell lysate
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab244395 has not yet been referenced specifically in any publications.