Key features and details
- Rabbit polyclonal to Deptor
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Deptor antibody
See all Deptor primary antibodies
DescriptionRabbit polyclonal to Deptor
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- ICC/IF: MCF7 cells. WB: Deptor overexpression HEK-293T cell lysate. IHC-P: Human skeletal muscle, fallopian tube and small intestine tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab244395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionNegative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes.
Sequence similaritiesContains 2 DEP domains.
Contains 1 PDZ (DHR) domain.
modificationsPhosphorylated. Phosphorylation weakens interaction with MTOR within mTORC1 and mTORC2.
- Information by UniProt
- DEP domain containing 6 antibody
- DEP domain-containing mTOR-interacting protein antibody
- DEP domain-containing protein 6 antibody
PFA-fixed, Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells stained for Deptor (green) using ab244395 at 4 µg/ml in ICC/IF.
Paraffin-embedded human small intestine tissue stained for Deptor using ab244395 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human fallopian tube tissue stained for Deptor using ab244395 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human skeletal muscle tissue stained for Deptor using ab244395 at 1/50 dilution in immunohistochemical analysis.
All lanes : Anti-Deptor antibody (ab244395) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : Deptor overexpression HEK-293T cell lysate
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244395 has not yet been referenced specifically in any publications.