Product nameAnti-DERL1/Derlin-1 antibody
See all DERL1/Derlin-1 primary antibodies
DescriptionRabbit polyclonal to DERL1/Derlin-1
Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Non human primates
Synthetic peptide within Human DERL1/Derlin-1 aa 201-251. The exact sequence is proprietary. NP_077271.1.
Database link: Q9BUN8
- HeLa, 293T and NIH3T3 whole cell lysate; Human ovarian carcinoma tissue
This product was previously labelled as DERL1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab176732 is affinity purified using an epitope specific to DERL1/Derlin-1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab176732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 29 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/50 - 1/250.|
RelevanceDerlin 1 is a channel-like protein found in the endoplasmic reticulum (ER) membrane. It is specifically required for the degradation of misfolded ER luminal proteins. Derlin 1 participates in the transfer of misfolded proteins from the ER to the cytosol, where they are destroyed by the proteasome in a ubiquitin-dependent manner. It may act by forming a channel that allows the retrotranslocation of misfolded proteins into the cytosol and then transferring them to the ATPase VCP complex, which translocates and ubiquitinates misfolded proteins. In the case of infection by cytomegaloviruses, derlin 1 plays a central role in export from the ER and subsequent degradation of MHC class I heavy chains via its interaction with US11 viral protein, which recognizes and associates with MHC class I heavy chains. Derlin 1 also participates in the degradation of misfolded cytomegalovirus US2 protein.
Cellular localizationEndoplasmic reticulum; multi pass membrane protein.
- DER 1 antibody
- DER1 antibody
- Der1 like protein 1 antibody
All lanes : Anti-DERL1/Derlin-1 antibody (ab176732) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 29 kDa
Exposure time: 10 seconds
ab176732 at 0.4 µg/ml detecting DERL1/Derlin-1 in HeLa whole cell lysate by WB following IP. ab176732 was used for IP at 3 µg/mg of lysate, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemical analysis of formalin fixed, paraffin embedded Human ovarian carcinoma tissue labeling DERL1/Derlin-1 using ab176732 at a 1/200 dilution, followed by peroxidase substrate detection.
ab176732 has not yet been referenced specifically in any publications.