Overview

  • Product name

    Anti-DERL1/Derlin-1 antibody
    See all DERL1/Derlin-1 primary antibodies
  • Description

    Rabbit polyclonal to DERL1/Derlin-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human DERL1/Derlin-1 aa 224-251.
    Sequence:

    PASMRRAADQNGGGGRHNWGQGFRLGDQ


    Database link: Q9BUN8

  • Positive control

    • WB: NIH/3T3 cell lysate. IHC-P: Human small intestine tissue. Flow cytometry: HeLa cells.
  • General notes

     This product was previously labelled as DERL1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab230491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000.
Flow Cyt 1/10 - 1/50.

Target

  • Relevance

    Derlin 1 is a channel-like protein found in the endoplasmic reticulum (ER) membrane. It is specifically required for the degradation of misfolded ER luminal proteins. Derlin 1 participates in the transfer of misfolded proteins from the ER to the cytosol, where they are destroyed by the proteasome in a ubiquitin-dependent manner. It may act by forming a channel that allows the retrotranslocation of misfolded proteins into the cytosol and then transferring them to the ATPase VCP complex, which translocates and ubiquitinates misfolded proteins. In the case of infection by cytomegaloviruses, derlin 1 plays a central role in export from the ER and subsequent degradation of MHC class I heavy chains via its interaction with US11 viral protein, which recognizes and associates with MHC class I heavy chains. Derlin 1 also participates in the degradation of misfolded cytomegalovirus US2 protein.
  • Cellular localization

    Endoplasmic reticulum; multi pass membrane protein.
  • Database links

  • Alternative names

    • DER 1 antibody
    • DER1 antibody
    • Der1 like protein 1 antibody
    • DERL 1 antibody
    • Derlin 1 antibody
    • DERtrin 1 antibody
    see all

Images

  • Anti-DERL1/Derlin-1 antibody (ab230491) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) cell extract at 35 µg

    Developed using the ECL technique.
  • Formalin-fixed, paraffin-embedded human small intestine tissue stained for DERL1/Derlin-1 with ab230491 at 1/50 dilution in immunohistochemical analysis.

  • Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling DERL1/Derlin-1 with ab230491 at 1/10 dilution (green) compared with a negative control (blue).

    FITC-conjugated goat-anti-rabbit secondary antibodies were used.

References

ab230491 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab230491.
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