Overview

  • Product name

  • Description

    Rabbit polyclonal to Desmin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Cow, Dog, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Desmin (internal sequence). The exact sequence is proprietary.
    Database link: P17661

  • Positive control

    • WB: Rat2, RD, A549 and HCT116 whole cell lysate; mouse muscle tissue lysate. IHC-P: Mouse smooth muscle tissue and skeletal muscle tissue; H1299 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/1000. Predicted molecular weight: 54 kDa.

Target

  • Function

    Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
  • Involvement in disease

    Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
    Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • CMD1I antibody
    • CSM1 antibody
    • CSM2 antibody
    • DES antibody
    • DESM_HUMAN antibody
    • Desmin antibody
    • FLJ12025 antibody
    • FLJ39719 antibody
    • FLJ41013 antibody
    • FLJ41793 antibody
    • Intermediate filament protein antibody
    • OTTHUMP00000064865 antibody
    see all

Images

  • Paraffin-embedded mouse smooth muscle tissue stained for Desmin with ab227224 at 1/500 dilution in immunohistochemical analysis.

  • Anti-Desmin antibody (ab227224) at 1/1000 dilution + Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 54 kDa



    10% SDS-PAGE

  • Anti-Desmin antibody (ab227224) at 1/500 dilution + RD (human rhabdomyosarcoma cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 54 kDa



    10% SDS-PAGE

  • All lanes : Anti-Desmin antibody (ab227224) at 1/3000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 54 kDa



    10% SDS-PAGE

  • Anti-Desmin antibody (ab227224) at 1/1000 dilution + Mouse muscle tissue lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 54 kDa



    10% SDS-PAGE

  • Paraffin-embedded mouse skeletal muscle tissue stained for Desmin with ab227224 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded NCI-H1299 (human lung carcinoma cell line) xenograft tissue stained for Desmin with ab227224 at 1/500 dilution in immunohistochemical analysis.

References

ab227224 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Carotid Artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6.0
Specification
Carotid Artery
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 22°C
Fixative
Paraformaldehyde

Le Zhen

Verified customer

Submitted Sep 18 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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