Anti-Desmin antibody (ab8592)
Key features and details
- Rabbit polyclonal to Desmin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Desmin antibody
See all Desmin primary antibodies -
Description
Rabbit polyclonal to Desmin -
Host species
Rabbit -
Specificity
In immunoblots, this antibody reacts with a 53 kD polypeptide. It does not react with vimentin or human cytokeratins. This antibody can be used to distinguish rhabdomyosarcomas and leiomyosarcomas from other soft tissue tumors, lymphomas, and carcinomas. -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Full length protein (Human).
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General notes
Prolonged fixation in buffered formalin can destroy the epitope.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.01% PBS, 0.2% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Mouse
Rat
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (5) |
Use at an assay dependent concentration. Detects a band of approximately 53 kDa.
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| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 53 kDa. |
Application notes
Is unsuitable for ICC/IF or IHC-P.
Target
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Function
Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. -
Involvement in disease
Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin. -
Sequence similarities
Belongs to the intermediate filament family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1674 Human
- Entrez Gene: 13346 Mouse
- Entrez Gene: 64362 Rat
- Omim: 125660 Human
- SwissProt: P17661 Human
- SwissProt: P31001 Mouse
- SwissProt: P48675 Rat
- Unigene: 594952 Human
see all -
Alternative names
- CMD1I antibody
- CSM1 antibody
- CSM2 antibody
see all
Images
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Western blot - Anti-Desmin antibody (ab8592)All lanes : Anti-Desmin antibody (ab8592) at 1/1000 dilution
Lane 1 : RMS13 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Human skeletal muscle tissue lysate
Lane 4 : Human heart tissue lysate
Lane 5 : C2C12 cell lysate
Lane 6 : Mouse skeletal muscle tissue lysate
Lane 7 : Mouse heart tissue lysate
Lane 8 : L6 cell lysate
Lane 9 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
Protocols
References (80)
ab8592 has been referenced in 80 publications.
- Rohn F et al. Impaired integrin a5 /ß1 -mediated hepatocyte growth factor release by stellate cells of the aged liver. Aging Cell 19:e13131 (2020). PubMed: 32157808
- Eid Mutlak Y et al. A signaling hub of insulin receptor, dystrophin glycoprotein complex and plakoglobin regulates muscle size. Nat Commun 11:1381 (2020). PubMed: 32170063
- Suzuki K et al. Neutrophil Elastase Damages the Pulmonary Endothelial Glycocalyx in Lipopolysaccharide-Induced Experimental Endotoxemia. Am J Pathol 189:1526-1535 (2019). PubMed: 31108101
- Findlay AR et al. Lithium chloride corrects weakness and myopathology in a preclinical model of LGMD1D. Neurol Genet 5:e318 (2019). PubMed: 31123706
- Boriushkin E et al. Shear-Stress Sensitive Inwardly-Rectifying K+ Channels Regulate Developmental Retinal Angiogenesis by Vessel Regression. Cell Physiol Biochem 52:1569-1583 (2019). PubMed: 31145841
