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    desmin-antibody-ab8592.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class III Desmin
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Anti-Desmin antibody (ab8592)

  • Datasheet
  • SDS
Reviews (16)Q&A (1)References (80)

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Western blot - Anti-Desmin antibody (ab8592)

    Key features and details

    • Rabbit polyclonal to Desmin
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-Desmin antibody
      See all Desmin primary antibodies
    • Description

      Rabbit polyclonal to Desmin
    • Host species

      Rabbit
    • Specificity

      In immunoblots, this antibody reacts with a 53 kD polypeptide. It does not react with vimentin or human cytokeratins. This antibody can be used to distinguish rhabdomyosarcomas and leiomyosarcomas from other soft tissue tumors, lymphomas, and carcinomas.
    • Tested Applications & Species

      Application Species
      WB
      Mouse
      Rat
      Human
      See all applications and species data
    • Immunogen

      Full length protein (Human).

    • General notes

      Prolonged fixation in buffered formalin can destroy the epitope.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.1% Sodium azide
      Constituents: 0.01% PBS, 0.2% BSA
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Intermediate Filaments
      • Class III
      • Desmin
      • Stem Cells
      • Mesenchymal Stem Cells
      • Myogenesis
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Extracellular matrix
      • Other
      • Cardiovascular
      • Heart
      • Cardiac arrhythmias

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab8592 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Mouse
    Rat
    Human
    Application Abreviews Notes
    WB (5)
    Use at an assay dependent concentration. Detects a band of approximately 53 kDa.
    Notes
    WB
    Use at an assay dependent concentration. Detects a band of approximately 53 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
    • Involvement in disease

      Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
      Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin.
    • Sequence similarities

      Belongs to the intermediate filament family.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession P17661 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1674 Human
      • Entrez Gene: 13346 Mouse
      • Entrez Gene: 64362 Rat
      • Omim: 125660 Human
      • SwissProt: P17661 Human
      • SwissProt: P31001 Mouse
      • SwissProt: P48675 Rat
      • Unigene: 594952 Human
      • Unigene: 6712 Mouse
      • Unigene: 39196 Rat
      see all
    • Alternative names

      • CMD1I antibody
      • CSM1 antibody
      • CSM2 antibody
      • DES antibody
      • DESM_HUMAN antibody
      • Desmin antibody
      • FLJ12025 antibody
      • FLJ39719 antibody
      • FLJ41013 antibody
      • FLJ41793 antibody
      • Intermediate filament protein antibody
      • OTTHUMP00000064865 antibody
      see all

    Images

    • Western blot - Anti-Desmin antibody (ab8592)
      Western blot - Anti-Desmin antibody (ab8592)
      All lanes : Anti-Desmin antibody (ab8592) at 1/1000 dilution

      Lane 1 : RMS13 cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : Human skeletal muscle tissue lysate
      Lane 4 : Human heart tissue lysate
      Lane 5 : C2C12 cell lysate
      Lane 6 : Mouse skeletal muscle tissue lysate
      Lane 7 : Mouse heart tissue lysate
      Lane 8 : L6 cell lysate
      Lane 9 : Rat heart tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size: 53 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking and dilution buffer: 5% NFDM/TBST.

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (80)

    Publishing research using ab8592? Please let us know so that we can cite the reference in this datasheet.

    ab8592 has been referenced in 80 publications.

    • Rohn F  et al. Impaired integrin a5 /ß1 -mediated hepatocyte growth factor release by stellate cells of the aged liver. Aging Cell 19:e13131 (2020). PubMed: 32157808
    • Eid Mutlak Y  et al. A signaling hub of insulin receptor, dystrophin glycoprotein complex and plakoglobin regulates muscle size. Nat Commun 11:1381 (2020). PubMed: 32170063
    • Suzuki K  et al. Neutrophil Elastase Damages the Pulmonary Endothelial Glycocalyx in Lipopolysaccharide-Induced Experimental Endotoxemia. Am J Pathol 189:1526-1535 (2019). PubMed: 31108101
    • Findlay AR  et al. Lithium chloride corrects weakness and myopathology in a preclinical model of LGMD1D. Neurol Genet 5:e318 (2019). PubMed: 31123706
    • Boriushkin E  et al. Shear-Stress Sensitive Inwardly-Rectifying K+ Channels Regulate Developmental Retinal Angiogenesis by Vessel Regression. Cell Physiol Biochem 52:1569-1583 (2019). PubMed: 31145841
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 17 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody

    Average
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab208572
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 07 2020

    Western blot abreview for Anti-Desmin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    20 µg
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 21 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-Desmin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue (Embryonic Mouse Heart Section)
    Permeabilization
    Yes - Tween-20
    Specification
    Embryonic Mouse Heart Section
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 25 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Desmin antibody

    Good
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Sample
    Mouse Cell (Pericytes (xenograft tumour tissue))
    Specification
    Pericytes (xenograft tumour tissue)
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 10 2013

    Immunohistochemistry (Frozen sections) abreview for Anti-Desmin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Colon)
    Specification
    Colon
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - triton 0,1% in PBS
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 24°C
    Read More

    Dr. Alessandro Gandelli

    Verified customer

    Submitted Mar 01 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody

    Good
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Intestine)
    Specification
    Intestine
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 28 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Desmin antibody

    Average
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Colon)
    Specification
    Colon
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - .1% triton x-100
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 19 2012

    Western blot abreview for Anti-Desmin antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (hek293)
    Loading amount
    50 µg
    Specification
    hek293
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 26 2010

    Immunohistochemistry - Free Floating abreview for Anti-Desmin antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (brain pericytes)
    Specification
    brain pericytes
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 15 2010

    Western blot abreview for Anti-Desmin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (muscle)
    Loading amount
    50 µg
    Specification
    muscle
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 27 2010

    1-10 of 17 Abreviews or Q&A

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