Overview

  • Product name
  • Description
    Rabbit polyclonal to Desmin
  • Host species
    Rabbit
  • Specificity
    In immunoblots, this antibody reacts with a 53 kD polypeptide. It does not react with vimentin or human cytokeratins. This antibody can be used to distinguish rhabdomyosarcomas and leiomyosarcomas from other soft tissue tumors, lymphomas, and carcinomas.
  • Tested applications
    Suitable for: Electron Microscopy, IP, ELISA, WB, IHC-Frmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Syrian hamster
  • Immunogen

    Full length protein (Human).

  • Positive control
    • Heart muscle, smooth and skeletal muscle, leiomyoma and rhabdomyomas
  • General notes
    Prolonged fixation in buffered formalin can destroy the epitope.

Properties

Applications

Our Abpromise guarantee covers the use of ab8592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy Use at an assay dependent concentration. PubMed: 17535849
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 53 kDa.
IHC-Fr Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
    • Involvement in disease
      Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
      Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin.
    • Sequence similarities
      Belongs to the intermediate filament family.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • CMD1I antibody
      • CSM1 antibody
      • CSM2 antibody
      • DES antibody
      • DESM_HUMAN antibody
      • Desmin antibody
      • FLJ12025 antibody
      • FLJ39719 antibody
      • FLJ41013 antibody
      • FLJ41793 antibody
      • Intermediate filament protein antibody
      • OTTHUMP00000064865 antibody
      see all

    Images

    • ab8592 was used to stain mouse prostate.

    • All lanes : Anti-Desmin antibody (ab8592) at 1/1000 dilution

      Lane 1 : RMS13 cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : Human skeletal muscle tissue lysate
      Lane 4 : Human heart tissue lysate
      Lane 5 : C2C12 cell lysate
      Lane 6 : Mouse skeletal muscle tissue lysate
      Lane 7 : Mouse heart tissue lysate
      Lane 8 : L6 cell lysate
      Lane 9 : Rat heart tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size: 53 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking and dilution buffer: 5% NFDM/TBST.

    References

    This product has been referenced in:
    • Wu X  et al. Baicalin inhibits PDGF-BB-induced hepatic stellate cell proliferation, apoptosis, invasion, migration and activation via the miR-3595/ACSL4 axis. Int J Mol Med 41:1992-2002 (2018). Read more (PubMed: 29393361) »
    • Rønning SB  et al. Vitamin K2 improves proliferation and migration of bovine skeletal muscle cells in vitro. PLoS One 13:e0195432 (2018). Read more (PubMed: 29617432) »
    See all 70 Publications for this product

    Customer reviews and Q&As

    11-16 of 16 Abreviews or Q&A

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (skeletal muscle)
    Specification
    skeletal muscle
    Gel Running Conditions
    Non-reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Mar 03 2009

    Application
    Immunoprecipitation
    Sample
    Mouse Cell lysate - whole cell (c2c12)
    Total protein in input
    100 µg
    Specification
    c2c12
    Immuno-precipitation step
    Protein A/G

    Abcam user community

    Verified customer

    Submitted Feb 24 2009

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (skeletal muscle)
    Loading amount
    30 µg
    Specification
    skeletal muscle
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Feb 24 2009

    Abreviews
    Application
    ELISA
    Sample
    Mouse Tissue sections (skeletal muscle lyse)
    Specification
    skeletal muscle lyse
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Type
    Sandwich (Capture)

    Abcam user community

    Verified customer

    Submitted Feb 18 2009

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Infarcted Heart)
    Specification
    Infarcted Heart
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%

    Dr. Mal Niladri

    Verified customer

    Submitted Nov 16 2006

    Question

    BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Non-specific staining. When we use a rabbit Ig G as negative control we are still seeing a strong signal similar to the "postive" sample. Please see attached slides. SAMPLE Cultured myocytes from dog and cat PRIMARY ANTIBODY Rabbit polyclonal to desmin - abcam (ab8592). Initial experiments diluted antibody in 1% BSA/PBS but latterly have diluted in 5% chick serum/PBS. Have titrated primary antibody through a range of 1:25 down to 1:200. Incubation time ranged from 1h at room temp to overnight at 4deg C. Washes initially consisted of PBS (3 x 5 minute washes)but latterly have lengthened the time of the washes and have included 0.05% Tween (which significantly improves the background staining)! DETECTION METHOD Fluorescent label Alexa Fluor 500 POSITIVE AND NEGATIVE CONTROLS USED No Positives tried (myocytes should express this protein) Negatives: PBS in place of primary or Rabbit IgG in place of primary ANTIBODY STORAGE CONDITIONS +4deg C FIXATION OF SAMPLE Add enough cold methanol to cover the cell layer. Incubate at -20?C for 10 minutes. Aspirate solution. Cover cell layer with cold acetone and incubate at -20?C for 1 minute. Aspirate solution. ANTIGEN RETRIEVAL None.We're looking at cultured cells. PERMEABILIZATION STEP None BLOCKING CONDITIONS We have tried a range of BSA concentrations and by increasing the blocking time. At the lat attaempt, we tried %5 chick serum. SECONDARY ANTIBODY Molecular probes.Goat anti-rabbit IgG antibody (Alexa Fluor 500)diluted in either 1%BSA/PBS and now latterly 5% chick serum/PBS.Rnage of secodary from 1:50 down to 1:5000. Secondary incubated for 1 hour at room temperature.Washes initially were for 3 x 5 min washes in PBS but now we wash for longer using 0.05% Tween in PBS. HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 7 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? The only differneces with the changes in the protocol is to reduce background fluorescence. We now are able to achieve a completely black background, but the neagative IgG fluoresces as musch as the positive antibody to desmin.

    Read More
    Answer

    Thank you for contacting us for technical support about ab8592 and taking the time to give your protocol details and your image. I have asked for the opinion of several colleagues and we all think that the staining you have with the antibody is actually correct and that you are experiencing staining with the isotype control because of the isotype control rather than the primary antibody or because of the blocking agents. May I please suggest you run the following control to check if indeed the problem is due to the isotype control or blocking agents: run in parallel - cells incubated with 10% normal goat serum 30min then with primary antibody and then with secondary antibody -cells incubated with 10% normal goat serum 30min, then with PBS only, then with secondary antibody. You may also want to change the detergent from Tween to TritonX100 (0.3%) in the dilution buffers and try to increase the specific signal by reducing fixation to 5 min, trying to fix with 4% paraformaldehyde 10min. In our experience the Molecular probes secondary antibodies can be diluted to 1:5000 and more with excellent results. I hope the above suggestions will help, please do not hesitate to contact me again if you still experience problems with those changes.

    Read More

    11-16 of 16 Abreviews or Q&A

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