Immunocytochemistry/ Immunofluorescence abreview for Anti-Desmin antibody

Good
Abreviews
This product is known to not work in this application or species.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (dermal fibroblasts)
Specification
dermal fibroblasts
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X-100
Blocking step
fetal calf serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C

Other product details

Dilution
1/80
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: 1% fetal calf serum

Secondary antibody

Name
Non-Abcam antibody was used: Alexa 488 anti rabbit
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Abcam user community

Verified customer

Submitted Feb 25 2010

Sign up