Western blot abreview for Anti-Desmin antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (muscle)
Loading amount
50 µg
Specification
muscle
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/2000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBST milk

Secondary antibody

Name
Non-Abcam antibody was used: anti rabbit
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/6000

Detection

Detection method
ECL+
Exposure
5 second(s)
Bands
Specific: 55 kDa

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Submitted Aug 27 2010

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