Immunohistochemistry - Free Floating abreview for Anti-Desmin antibody

Below Average
Abreviews
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (brain pericytes)
Specification
brain pericytes

Other product details

Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% NGS, 0.2% Triton X, in PBS

Secondary antibody

Name
Non-Abcam antibody was used: goat anti rabbit Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Additional data

Additional Notes
Secondary was incubated for 6h at RT.

Mice perfused with 4% paraformaldehyde, brains were extracted, postfixed for 2 days, then incubated in 30% sucrose, embedded and cut.

Desmin only labelled along very large blood vessels, which suggests it is labelling smooth muscle cells but not pericytes.
Abcam response
This product is being used in an untested application. Based on this Abreview this product may not be appropriate for use in this application.

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Submitted Oct 15 2010

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