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Immunohistochemistry free floating
Mouse Tissue sections (brain pericytes)
Other product details
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% NGS, 0.2% Triton X, in PBS
Non-Abcam antibody was used: goat anti rabbit Alexa Fluor 488
Host species: Goat
Conjugation: Alexa Fluor® 488
Secondary was incubated for 6h at RT.
Mice perfused with 4% paraformaldehyde, brains were extracted, postfixed for 2 days, then incubated in 30% sucrose, embedded and cut.
Desmin only labelled along very large blood vessels, which suggests it is labelling smooth muscle cells but not pericytes.
This product is being used in an untested application. Based on this Abreview this product may not be appropriate for use in this application.
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Submitted Oct 15 2010