Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
Key features and details
- Rabbit polyclonal to Desmin - Cytoskeleton Marker
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Desmin antibody - Cytoskeleton Marker
See all Desmin primary antibodies -
Description
Rabbit polyclonal to Desmin - Cytoskeleton Marker -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Desmin aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P17661 -
Positive control
- ICC/IF: RMS13 and C2C12 cells. WB: Human placenta tissue lysate. IHC-P: Human skeletal muscle and human and rat cardiac muscle tissue.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The negative control used to test this Ab was: skin(squamous epithelial cells)was used as negative control tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab15200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 52 kDa. | |
| IHC-P | 1/200. |
Target
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Function
Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. -
Involvement in disease
Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin. -
Sequence similarities
Belongs to the intermediate filament family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1674 Human
- Entrez Gene: 13346 Mouse
- Entrez Gene: 64362 Rat
- Omim: 125660 Human
- SwissProt: P17661 Human
- SwissProt: P31001 Mouse
- SwissProt: P48675 Rat
- Unigene: 594952 Human
see all -
Alternative names
- CMD1I antibody
- CSM1 antibody
- CSM2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)Immunohistochemical analysis of paraffin-embedded on human skeletal muscle labeling Desmin with ab15200 at 1/200 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). Counter stained with hematoxylin.
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Immunocytochemistry/ Immunofluorescence - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)Immunocytochemistry/Immunofluorescence analysis of C2C12 (mouse muscle) cells labelling Desmin with ab15200 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
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Immunocytochemistry/ Immunofluorescence - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)This image is courtesy of an Abreview submitted by Dr Mal Niladriab15200 staining rat differentiated skeletal muscle cells by ICC/IF.
Cultured differentiated skeletal muscle cells were harvested from Lewis rats, 2% paraformaldehyde treated for 15 min to fix the cells, and then permealized with Triton-X100 (0.1%) for 10 min. The cells were then incubated with ab15200 at 1/200 overnight at 4°C. The image was taken with a confocal laser scanning microscope and shows desmin expressing skeletal myocytes (green-cytoplasmic localization). Note that desmin forms short thickened filamentous structures in the cell cytoplasm, along with prominent spot-like cytoplasmic aggregates that are composed of densely packed filaments.
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Immunocytochemistry/ Immunofluorescence - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)Immunocytochemistry/Immunofluorescence analysis of RMS13 (human rhabdomyosarcoma) cells labelling Desmin with ab15200 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
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Immunocytochemistry/ Immunofluorescence - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)Immunocytochemistry/Immunofluorescence analysis of A431 (human epidermoid carcinoma) cells (negative cell line) showing weak staining of Desmin with ab15200 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
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Immunocytochemistry/ Immunofluorescence - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)This image is courtesy of an anonymous abreview.ab15200 staining Desmin in rat Smooth muscle cells from mesenteric artery by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 4% paraformaldehyde in PSS for 4 min at 4°C and permeabilized with 0.3% Triton x100 before blocking with 2% BSA was done for 30 minutes at 20°C. Samples were incubated with primary antibody (1/300 in PSS with 2%BSA and 0.3% Triton X-100) for 14 hours at 4°C. An MFP 555-conjugated donkey polyclonal to rabbit IgG was used as secondary antibody at 1/400 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)Immunohistochemical analysis of paraffin-embedded on human cardiac muscle labeling Desmin with ab15200 at 1/200 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). Counter stained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)Immunohistochemical analysis of paraffin-embedded on rat cardiac muscle labeling Desmin with ab15200 at 1/200 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). Counter stained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)This image is courtesy of an anonymous abreview.ab15200 staining Desmin in Mouse skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature; antigen retrieval was enzymatic. Samples were incubated with primary antibody (1/800 in blocking buffer) for 16 hours at 4°C. An undiluted HRP-conjugated Horse anti-rabbit IgG polyclonal was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)Ab15200 positively staining desmin in formaldehyde fixed mouse heart tissue (1/200). Ab15200 was used in conjunction with goat anti rabbit (biotin).
The reviewer reported no difference in the quality of staining obtained when enzymatic epitope unmasking was preformed.
This image is an edited version of an image submitted courtesy of an Abreview by Khaled Chatila on 11 October 2005. For further information relating to the protocol please refer to the Abreview. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)This image is courtesy of an anonymous abreview.Immunohistochemical analysis of PFA-fixed paraffin-embedded murine nephric tissue, labelling Desmin with ab15200 at a dilution of 1/250 incubated for 18 hours at 4°C. Heat mediated antigen retrival was with citrate buffer. Blocking was with Dako serum-free protein block at 100% incubated for 1 hour at 23°C. Secondary was a donkey anti-rabbit polyclonal HRP conjugate at 1/200.
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Western blot - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)Anti-Desmin antibody - Cytoskeleton Marker (ab15200) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 52 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 72 kDa (possible non-specific binding)
Protocols
References
ab15200 has been referenced in 123 publications.
- Bella P et al. Blockade of IGF2R improves muscle regeneration and ameliorates Duchenne muscular dystrophy. EMBO Mol Med 12:e11019 (2020). PubMed: 31793167
- Dai J et al. A necroptotic-independent function of MLKL in regulating endothelial cell adhesion molecule expression. Cell Death Dis 11:282 (2020). PubMed: 32332696
- Morrell MBG et al. Vascular modulation through exercise improves chemotherapy efficacy in Ewing sarcoma. Pediatr Blood Cancer 66:e27835 (2019). PubMed: 31136074
- Bonnardel J et al. Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage Niche. Immunity 51:638-654.e9 (2019). PubMed: 31561945
- Liu B et al. Zhen-wu-tang ameliorates membranous nephropathy rats through inhibiting NF-?B pathway and NLRP3 inflammasome. Phytomedicine 59:152913 (2019). PubMed: 30991182
