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  1. Link

    desmin-antibody-cytoskeleton-marker-ab15200.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class III Desmin
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Anti-Desmin antibody - Cytoskeleton Marker (ab15200)

  • Datasheet
  • SDS
Reviews (26)Q&A (6)References (139)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
  • Western blot - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)

Key features and details

  • Rabbit polyclonal to Desmin - Cytoskeleton Marker
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Anti-CD31 antibody (ab28364)

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Overview

  • Product name

    Anti-Desmin antibody - Cytoskeleton Marker
    See all Desmin primary antibodies
  • Description

    Rabbit polyclonal to Desmin - Cytoskeleton Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Desmin aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P17661

  • Positive control

    • ICC: ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), C2C12, RMS13, and A431 cells. IHC-P: Human skeletal muscle, human and rat cardiac muscle. WB: Human placenta tissue lysate.
  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The negative control used to test this Ab was: skin(squamous epithelial cells)was used as negative control tissue.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class III
    • Desmin
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other
    • Cardiovascular
    • Heart
    • Cardiac arrhythmias
    • Signal Transduction
    • Antibodies
    • desmin

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612)

Applications

Our Abpromise guarantee covers the use of ab15200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 0.1 - 1 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.
IHC-P 1/200.

Target

  • Function

    Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
  • Involvement in disease

    Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
    Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P17661 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1674 Human
    • Entrez Gene: 13346 Mouse
    • Entrez Gene: 64362 Rat
    • Omim: 125660 Human
    • SwissProt: P17661 Human
    • SwissProt: P31001 Mouse
    • SwissProt: P48675 Rat
    • Unigene: 594952 Human
    • Unigene: 6712 Mouse
    • Unigene: 39196 Rat
    see all
  • Alternative names

    • CMD1I antibody
    • CSM1 antibody
    • CSM2 antibody
    • DES antibody
    • DESM_HUMAN antibody
    • Desmin antibody
    • FLJ12025 antibody
    • FLJ39719 antibody
    • FLJ41013 antibody
    • FLJ41793 antibody
    • Intermediate filament protein antibody
    • OTTHUMP00000064865 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)

    Immunohistochemical analysis of paraffin-embedded on human skeletal muscle labeling Desmin with ab15200 at 1/200 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). Counter stained with hematoxylin.

  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)

    Immunofluorescence staining of Desmin using ab15200 in ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), which were differentiated for 10 days post induction.


    The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab15200 at 0.1 µg/mL and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin, at 1/1000 dilution. Cells were then incubated with ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).


    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.


    The antibody ab15200 gave comparable results using MeOH fixation (100%, 5 min).

  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)

    Immunocytochemistry/Immunofluorescence analysis of C2C12 (mouse muscle) cells labelling Desmin with ab15200 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)This image is courtesy of an Abreview submitted by Dr Mal Niladri

    ab15200 staining rat differentiated skeletal muscle cells by ICC/IF.

    Cultured differentiated skeletal muscle cells were harvested from Lewis rats, 2% paraformaldehyde treated for 15 min to fix the cells, and then permealized with Triton-X100 (0.1%) for 10 min. The cells were then incubated with ab15200 at 1/200 overnight at 4°C. The image was taken with a confocal laser scanning microscope and shows desmin expressing skeletal myocytes (green-cytoplasmic localization). Note that desmin forms short thickened filamentous structures in the cell cytoplasm, along with prominent spot-like cytoplasmic aggregates that are composed of densely packed filaments.

    See Abreview

  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)

    Immunocytochemistry/Immunofluorescence analysis of RMS13 (human rhabdomyosarcoma) cells labelling Desmin with ab15200 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)

    Immunocytochemistry/Immunofluorescence analysis of A431 (human epidermoid carcinoma) cells (negative cell line) showing weak staining of Desmin with ab15200 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)This image is courtesy of an anonymous abreview.

    ab15200 staining Desmin in rat Smooth muscle cells from mesenteric artery by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 4% paraformaldehyde in PSS for 4 min at 4°C and permeabilized with 0.3% Triton x100 before blocking with 2% BSA was done for 30 minutes at 20°C. Samples were incubated with primary antibody (1/300 in PSS with 2%BSA and 0.3% Triton X-100) for 14 hours at 4°C. An MFP 555-conjugated donkey polyclonal to rabbit IgG was used as secondary antibody at 1/400 dilution.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)

    Immunohistochemical analysis of paraffin-embedded on human cardiac muscle labeling Desmin with ab15200 at 1/200 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). Counter stained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)

    Immunohistochemical analysis of paraffin-embedded on rat cardiac muscle labeling Desmin with ab15200 at 1/200 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). Counter stained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)This image is courtesy of an anonymous abreview.

    ab15200 staining Desmin in Mouse skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature; antigen retrieval was enzymatic. Samples were incubated with primary antibody (1/800 in blocking buffer) for 16 hours at 4°C. An undiluted HRP-conjugated Horse anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Ab15200 positively staining desmin in formaldehyde fixed mouse heart tissue (1/200). Ab15200 was used in conjunction with goat anti rabbit (biotin).

    The reviewer reported no difference in the quality of staining obtained when enzymatic epitope unmasking was preformed.

    This image is an edited version of an image submitted courtesy of an Abreview by Khaled Chatila on 11 October 2005. For further information relating to the protocol please refer to the Abreview.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)This image is courtesy of an anonymous abreview.

    Immunohistochemical analysis of PFA-fixed paraffin-embedded murine nephric tissue, labelling Desmin with ab15200 at a dilution of 1/250 incubated for 18 hours at 4°C. Heat mediated antigen retrival was with citrate buffer. Blocking was with Dako serum-free protein block at 100% incubated for 1 hour at 23°C. Secondary was a donkey anti-rabbit polyclonal HRP conjugate at 1/200.

    See Abreview

  • Western blot - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Western blot - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Anti-Desmin antibody - Cytoskeleton Marker (ab15200) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 52 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 72 kDa (possible non-specific binding)

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (139)

    Publishing research using ab15200? Please let us know so that we can cite the reference in this datasheet.

    ab15200 has been referenced in 139 publications.

    • Bella P  et al. Blockade of IGF2R improves muscle regeneration and ameliorates Duchenne muscular dystrophy. EMBO Mol Med 12:e11019 (2020). PubMed: 31793167
    • Dai J  et al. A necroptotic-independent function of MLKL in regulating endothelial cell adhesion molecule expression. Cell Death Dis 11:282 (2020). PubMed: 32332696
    • Abdelmoez AM  et al. Comparative profiling of skeletal muscle models reveals heterogeneity of transcriptome and metabolism. Am J Physiol Cell Physiol 318:C615-C626 (2020). PubMed: 31825657
    • Xie L  et al. Panax Notoginseng Ameliorates Podocyte EMT by Targeting the Wnt/ß-Catenin Signaling Pathway in STZ-Induced Diabetic Rats. Drug Des Devel Ther 14:527-538 (2020). PubMed: 32103895
    • Hoffmann C  et al. Hepatic stellate cell hypertrophy is associated with metabolic liver fibrosis. Sci Rep 10:3850 (2020). PubMed: 32123215
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 32 Abreviews or Q&A

    Question

    Could you suggest secondary antibody for antiDesmin antibody ab15200.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 30 2012

    Answer

    Thank you for your inquiry.

    We have a number of antibodies in our catalogue that might be of interest to you. I would like to direct you to the search facility on our website (www.abcam.com).

    Please click the secondary antibody tab at the top of the home page. A advance search will be generated which can be further refined using the filters on the page. Using these filters will help you to identify products that have been tested in the applications and species you are interested in.

    As we discussed on phone; please check the following link for free prism ladder;

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    I hope this information is helpful to you. If you have specific questions about the products identified during the search, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Apr 30 2012

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Desmin antibody - Cytoskeleton Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Pig Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 100x Citrate Buffer pH 6.0(ab64236)
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 30 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Desmin antibody - Cytoskeleton Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 16 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Desmin antibody - Cytoskeleton Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 25 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody - Cytoskeleton Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    liver
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 13 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody - Cytoskeleton Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Colon
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 13 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody - Cytoskeleton Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (heart)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    heart
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 13 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody - Cytoskeleton Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (heart)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    heart
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 13 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody - Cytoskeleton Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 13 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Desmin antibody - Cytoskeleton Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (kidney H9 organoids)
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    kidney H9 organoids
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 13 2020

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