Product nameAnti-Desmin antibody [DE-U-10] - Cytoskeleton Marker
See all Desmin primary antibodies
DescriptionMouse monoclonal [DE-U-10] to Desmin - Cytoskeleton Marker
Tested applicationsSuitable for: WB, IHC-FoFr, IHC-P, IHC-Frmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Rabbit, Goat, Chicken, Hamster, Cow, Cat, Lizard, Snake
Full length native protein (purified) (Pig).
- This antibody gave a positive result in IHC in the following FFPE tissue: Human Skeletal muscle.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
PurityProtein G purified
Light chain typeunknown
Our Abpromise guarantee covers the use of ab6322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.
Monoclonal anti-desmin may be used for Immunocytochemical localization of intermediate filaments of the desmin group in all types of muscle cells and to localize desmin at the periphery of z-discs.
|IHC-FoFr||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionDesmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
Involvement in diseaseDefects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin.
Sequence similaritiesBelongs to the intermediate filament family.
- Information by UniProt
- CMD1I antibody
- CSM1 antibody
- CSM2 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue labelling Desmin with ab6322 at a dulution of 1/200. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with Hematoxylin.
Image shows cytomplasmic staining on mouse cardiac muscle.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue labelling Desmin with ab6322 at a dulution of 1/200. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with Hematoxylin.
Image shows cytomplasmic staining on smooth muscle of rat colon.
Western blot analysis of human skeletal muscle whole tissue lysate (2µg/lane) labelling Desmin with ab6322 at a dilution of 1/2000 in TBS for 18 hours at 4°C.
Blocked in 5% milk for 2 hours at 25°C.
A IRDye 680RD polyclonal goat anti-mouse IgG was used as the secondary antibody at a dilution of 1/5000.
Immunohistochemical analysis of Human colorectal tissue, staining Desmin (green) with ab6322.
Tissue was fixed with ice cold acetone for 5 minutes and blocked with 10% goat serum for 30 minutes. Samples were incubated with primary antibody (1/30) overnight at 4°C and an AlexaFluor®488®-conjugated anti-mouse IgG (1/500) was used as the secondary antibody.
Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322) at 1/3000 dilution + Mouse skeletal muscle tissue lysate at 30 µg
HRP-conjugated Goat anti Mouse IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
Blocked with 5% Milk for 1 hour at 25°C.
ab6322 at 1/100 staining rabbit oesophagus tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum before incuabion with the antibody for 1 hour. An Alexa-Fluor ® 594 conjugated goat anti-mouse IgG1 was used as the secondary.
IHC image of Desmin staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6322, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
This product has been referenced in:
- Dye DE et al. Interaction Between Skeletal Muscle Cells and Extracellular Matrix Proteins Using a Serum Free Culture System. Methods Mol Biol 1889:185-212 (2019). Read more (PubMed: 30367415) »
- Estañ MC et al. Recessive mutations in muscle-specific isoforms of FXR1 cause congenital multi-minicore myopathy. Nat Commun 10:797 (2019). Read more (PubMed: 30770808) »