Overview

  • Product name
    Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker
    See all Desmin primary antibodies
  • Description
    Mouse monoclonal [DE-U-10] to Desmin - Cytoskeleton Marker
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-FoFr, IHC-P, IHC-Frmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Goat, Chicken, Hamster, Cow, Cat, Lizard, Snake
  • Immunogen

    Full length native protein (purified) (Pig).

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human Skeletal muscle.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab6322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.

Monoclonal anti-desmin may be used for Immunocytochemical localization of intermediate filaments of the desmin group in all types of muscle cells and to localize desmin at the periphery of z-discs.

IHC-FoFr Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
    • Involvement in disease
      Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
      Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin.
    • Sequence similarities
      Belongs to the intermediate filament family.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • CMD1I antibody
      • CSM1 antibody
      • CSM2 antibody
      • DES antibody
      • DESM_HUMAN antibody
      • Desmin antibody
      • FLJ12025 antibody
      • FLJ39719 antibody
      • FLJ41013 antibody
      • FLJ41793 antibody
      • Intermediate filament protein antibody
      • OTTHUMP00000064865 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue labelling Desmin with ab6322 at a dulution of 1/200. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with Hematoxylin.

      Image shows cytomplasmic staining on mouse cardiac muscle.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue labelling Desmin with ab6322 at a dulution of 1/200. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with Hematoxylin.

      Image shows cytomplasmic staining on smooth muscle of rat colon.

    • Western blot analysis of human skeletal muscle whole tissue lysate (2µg/lane) labelling Desmin with ab6322 at a dilution of 1/2000 in TBS for 18 hours at 4°C.

      Blocked in 5% milk for 2 hours at 25°C.

      A IRDye 680RD polyclonal goat anti-mouse IgG was used as the secondary antibody at a dilution of 1/5000.

      See Abreview

    • Immunohistochemical analysis of Human colorectal tissue, staining Desmin (green) with ab6322.

      Tissue was fixed with ice cold acetone for 5 minutes and blocked with 10% goat serum for 30 minutes. Samples were incubated with primary antibody (1/30) overnight at 4°C and an AlexaFluor®488®-conjugated anti-mouse IgG (1/500) was used as the secondary antibody.
    • Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322) at 1/3000 dilution + Mouse skeletal muscle tissue lysate at 30 µg

      Secondary
      HRP-conjugated Goat anti Mouse IgG polyclonal at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Observed band size: 55 kDa why is the actual band size different from the predicted?


      Exposure time: 2 minutes


      Blocked with 5% Milk for 1 hour at 25°C.

      See Abreview

    • ab6322 at 1/100 staining rabbit oesophagus tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum before incuabion with the antibody for 1 hour. An Alexa-Fluor ® 594 conjugated goat anti-mouse IgG1 was used as the secondary.

      See Abreview

    • IHC image of Desmin staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6322, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    Protocols

    References

    This product has been referenced in:
    • Dye DE  et al. Interaction Between Skeletal Muscle Cells and Extracellular Matrix Proteins Using a Serum Free Culture System. Methods Mol Biol 1889:185-212 (2019). Read more (PubMed: 30367415) »
    • Estañ MC  et al. Recessive mutations in muscle-specific isoforms of FXR1 cause congenital multi-minicore myopathy. Nat Commun 10:797 (2019). Read more (PubMed: 30770808) »
    See all 32 Publications for this product

    Customer reviews and Q&As

    1-8 of 8 Q&A

    Answer

    I have issued a free of charge replacement with a new lot.

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    Answer

    Thank you for sending your protocol, which appears to be correct, and the image file. I agree that the antibody is staining something that is unlikely to be desmin. Are you interested in trying a different antibody against desmin? I see that ab15200 has been published in an article that used it to identify pericytes:

    Schadler KL et al. Delta-like ligand 4 plays a critical role in pericyte/vascular smooth muscle cell formation during vasculogenesis and tumor vessel expansion in Ewing's sarcoma. Clin Cancer Res 16:848-56 (2010). PubMed: 20103680

    Here is the link to the PubMed abstract:

    http://www.ncbi.nlm.nih.gov/pubmed/20103680?dopt=Abstract

    and a link to the antibody datasheet:

    https://www.abcam.com/Desmin-antibody-ab15200.html

    I will be happy to send this as a free-of-charge replacement for ab6322, or a refund if you prefer. Please let me know how you would like to proceed.

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    Answer

    Thank you for your enquiry whichi has been forwarded to the scientific support team. I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications and species listed on the datasheets. Therefore, any Desmin antibody tested in the species and application you are using should be suitable and guaranteed for your experiments. I am sorry to confirm these antibodies have not specifically been tested in human hemaetopoitic progenitor cells. However, I am pleased to let you know that both ab15200 and ab6322 have been tested on human samples and would be covered by our guarantee for use on samples from this species. I can recommend to check the datasheet to view a list of the tested applications to ensure that the application you are using is also tested and guaranteed. The price of products can be found at the top of the datasheet, please ensure to select Singapore from the country selection at the top right hand corner of the page to see the correct price. ab15200 tested in: Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB Price USD $365.00 Click here (or use the following: https://www.abcam.com/index.html?datasheet=15200). ab6322 tested in: ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB Price USD $365.00 Click here (or use the following: https://www.abcam.com/index.html?datasheet=6322). Freight/Packing: USD $60.00 to Singapore Although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done. If you wish to be more certain, I can suggest choosing the antibody that has been best characterized. By this I mean the antibody that has been tested in the most applications and has the most data available (on the datasheet). I hope this will be helpful to you. Should you have any further questions please do not hesitate to ask.

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    Question
    Answer

    Thanks for your call today. Please see below for your testing discount codes and instructions for use. Let me know if you have any questions! DISCOUNT CODES: (1) ab15463: *** (2) ab6322: *** Expiration date: January 22, 2012 I am very pleased to hear you would like to accept our offer and test these antibodies in rhesus monkey. Each code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for each antibody in rhesus samples and include the code in the “Additional Comments” section so we know the Abreview is for this promotion. The codes will be active once the Abreviews have been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreviews and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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    Answer

    Unfortunately, we do not sell this antibody or any other Desmin antibody in conjugated version. I do not have the information at the moment if and when a conjugated version will be added to the catalogue.

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    Answer

    Thank you for your patience. I'm sorry to hear that your customer is experiencing trouble with this antibody. We do not know if this antibody works on Bouin's fixed tissues, however it has definitely been tested and characterized for formalin-fixed paraffin-embedded sections, so I would suggest that your customer try that type of fixation. Also, the antibody has been tested for cross-reactivity with chicken, but has not yet tested for cross-reactivity with turtle. Is your customer doing antigen retrieval? On the online datasheet for this antibody there is a protocol listed for antigen retrieval listed in the protocols tab. I hope that this is useful and if you have any more questions, please contact us again.

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    Answer

    Thank you for bringing this matter to our attention. We are pleased that you are happy with the product quality and speed of delivery. However, there should indeed be 100µl in the vial and you should not experience any problems with excessive bubbles. The antibody is supplied as ascitic fluid with 15mM sodium azide. Please let me know when you place another order with us and I shall arrange for a discount to compensate for the shortfall in volume. In the meantime, please accept my apologies.

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    Answer

    This antibody was raised against the whole molecule, and so we do not know the epitope.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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