Western blot abreview for Anti-Desmin antibody [DE-U-10]

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (skeletal muscle)
Loading amount
30 µg
Specification
skeletal muscle
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/3000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 20 mM Tris-HCl, 150 mM NaCl, pH 7.4

Secondary antibody

Name
Non-Abcam antibody was used: anti-mouse Ig
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 55 kDa

Additional data

Additional Notes
protein band above 100 kDa might be desmin dimer

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Submitted Jun 14 2012

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