Western blot abreview for Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker

Excellent
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Tissue lysate - whole (skeletal muscle)
Specification
skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS
Dilution
1/2000

Secondary antibody

Name
Non-Abcam antibody was used: IRDye 680RD Goat (polyclonal) Anti-Mouse IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye­ 680
Dilution
1/5000

Detection

Detection method
Odyssey
Bands
Specific: 53 kDa
Exposure
1 second(s)

Additional data

Additional Notes
There is not exposition time when using Odissey

Dr. Rafael Montes Nieto

Verified customer

Submitted Oct 31 2014

Sign up