Immunocytochemistry/ Immunofluorescence abreview for Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker

Inconclusive
Abreviews
This product is known to not work in this application or species.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (muscle)
Permeabilization
Yes - PBS triton0.5%
Specification
muscle
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 20°C
Fixative
Paraformaldehyde

Other product details

Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS Triton 0.5% BSA 1%
Dilution
1/20

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Invitrogen alexa fluor anti-mouse 488
Host species: Mouse
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
We have tested this Ab on human immortalized myoblasts and on C2C12. Try several protocols but nothing seems to work
Abcam response
We recently found this antibody unsuitable for ICC or ICC/IF and do not guarantee it in this application.

Abcam user community

Verified customer

Submitted Mar 14 2016

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