Question (20013) | Anti-Desmin antibody [RD301] (ab8976)

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Question

BATCH NUMBER 22587 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM we did immunhistos with two of your lots. lot number 25677 gave a very nice Z-disc and intercalated disc staining. we used ist 1:200. Lot number 22587 did not give any signal at all. Two different persons used it at dilution 1:100 and 1:200. SAMPLE Mouse heart, frozen sections. PRIMARY ANTIBODY Desmin (see above) DETECTION METHOD Fluorescence microscop POSITIVE AND NEGATIVE CONTROLS USED as negative control we used only the second antibody. As positive control we used a second antibody in order to colocalize it with desmin. That worked.ANTIBODY STORAGE CONDITIONS -20°C FIXATION OF SAMPLE we used both : actone (-20°C) and Paraformaldehyde. It did not work on either sections. The other lot worked with acetone fixation. BLOCKING CONDITIONS BSA 2% in PBS for 1 hour SECONDARY ANTIBODY FITC anti-mouse Vector HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? We used different dilution and fixation methods. But after finding out that lot number 25677 works we did not do more tests. ADDITIONAL NOTES I could send you the pictures I have taken that have worked. In this case please just write me an email.

Answer

Thank you for contacting us for technical support regarding your recent problem with a vial of ab8976. I'm sorry to hear you are experiencing problems with this vial and I would appreciate if you could please clarify which vial is not working for you. According to your orders you have received two tubes: lot 256477 is the most recent one (the one you said worked well) was ordered a month ago lot 225987 was ordered in December 2006 (the one you said did not work). Is this correct or is it the opposite happening i.e. the new vial is not working? Thank you very much for clarifying, I look forward to hearing from you to resolve this matter,

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