Name: Anti-Desmin antibody [Y66] - Cytoskeleton Marker
Brand: RabMAb
SKU: ab32362
Rating: 5 (12 reviews)

Product name

Anti-Desmin antibody [Y66] - Cytoskeleton Marker
See all Desmin primary antibodies
Description

Rabbit monoclonal [Y66] to Desmin - Cytoskeleton Marker
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, WB, IHC-P, IHC - Wholemount, Flow Cyt, IHC-Frmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Guinea pig, Human
Immunogen

Synthetic peptide within Human Desmin aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
Epitope

ab32362 reacts with an epitope located in the C terminal region of desmin.
Positive control
- WB: Human skeletal muscle, fetal heart and fetal muscle tissue lysates. Mouse and rat heart tissue lysates. Guinea pig heart and muscle tissue lysates. ICC/IF: A673 cells. IHC-P: Human skeletal muscle, uterus and urinary bladder tissues. Flow Cyt: C2C12 and HeLa cells.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

Y66
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab32362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC/IF		1/50.
WB		1/100000. Predicted molecular weight: 53 kDa.
IHC-P		1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
IHC - Wholemount		Use at an assay dependent concentration. PubMed: 21856924
Flow Cyt		1/70. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr		Use at an assay dependent concentration. PubMed: 21824387

Application notes

Is unsuitable for IP.

Function

Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
Involvement in disease

Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin.
Sequence similarities

Belongs to the intermediate filament family.
Cellular localization

Cytoplasm.
Target information above from: UniProt accession P17661 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 1674 Human
- Entrez Gene: 13346 Mouse
- Entrez Gene: 64362 Rat
- Omim: 125660 Human
- SwissProt: P17661 Human
- SwissProt: P31001 Mouse
- SwissProt: P48675 Rat
- Unigene: 594952 Human
- Unigene: 6712 Mouse
- Unigene: 39196 Rat
see all
Alternative names
- CMD1I antibody
- CSM1 antibody
- CSM2 antibody
- DES antibody
- DESM_HUMAN antibody
- Desmin antibody
- FLJ12025 antibody
- FLJ39719 antibody
- FLJ41013 antibody
- FLJ41793 antibody
- Intermediate filament protein antibody
- OTTHUMP00000064865 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling Desmin with purified ab32362 at 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)This image is courtesy of an anonymous Abreview

Unpurified ab32362 staining Desmin (green) in Human skeletal muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methacarn and blocked with 10% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/150) for 12 hours. An Alexa Fluor^® 488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Blue - DAPI-nuclei. Red - WGA. 40X objective.
See Abreview
Western blot - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

All lanes : Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362) at 1/500000 dilution (purified)

Lane 1 : Human skeletal muscle tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : Human fetal muscle tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 53 kDa
Observed band size: 53 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Flow Cytometry - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

Flow Cytometry analysis of C2C12 cells labelling Desmin with purified ab32362 at 1/70 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Immunocytochemistry/ Immunofluorescence - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

Immunocytochemistry/Immunofluorescence analysis of A673 cells labelling Desmin with purified ab32362 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Control: primary antibody (1/50) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)Cowan DB et al. Intracoronary Delivery of Mitochondria to the Ischemic Heart for Cardioprotection. PLoS One 11:e0160889 (2016).

Immunofluorescent analysis of Human mitochondria injected rabbit hearts sections stained for Desmin (Green) using ab32362. MTCO2, the human-specific mitochondrial marker was stained in red, and the nuclei was stained using the DNA stain DAPI (blue).
Western blot - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

All lanes : Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362) at 1/500000 dilution (purified)

Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat heart tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 53 kDa
Observed band size: 53 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

All lanes : Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362) at 1/100000 dilution

Lane 1 : Guinea pig heart tissue lysate
Lane 2 : Guinea pig muscle tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 53 kDa
Observed band size: 53 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunohistochemistry (Frozen sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)Image from Alexander MS et al., Skelet Muscle. 2011 Aug 8;1:27. Fig S4.; doi:10.1186/2044-5040-1-27; 8 August 2011, Skeletal Muscle 2011, 1:27

Immunohistochemistry (Frozen sections) analysis of mouse skeletal muscle tissue following cardiotoxin injury, labelling Desmin with unpurified ab32362.
Western blot - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)This image is courtesy of an anonymous Abreview

All lanes : Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362) at 1/500 dilution (unpurified)

All lanes : Human skeletal muscle whole tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : IRDye® 680-conjugated anti-rabbit at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 53 kDa
Observed band size: 53 kDa

Exposure time: 50 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of normal human uterus tissue labelling Desmin with unpurified ab32362.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of normal human urinary bladder tissue labelling Desmin with unpurified ab32362.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human skeletal muscle tissue labelling Desmin with unpurified ab32362.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of normal human brain tissue. Unpurified ab32362 shows negative staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of normal human tonsil tissue. Unpurified ab32362 shows negative staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)Image courtesy of an anonymous Abreview.

Unpurified ab32362 staining Desmin in nude rat esophagheal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6. Samples were then blocked with 1% BSA for 20 minutes at 25°C and then incubated with unpurified ab32362 at a 1/400 dilution for 16 hours at 25°C. The secondary used was an undiluted goat anti-rabbit HRP conjugated polyclonal.Striated muscle cells of muscular propria are strongly positive for desmin. Smooth muscle cells and vascular smooth muscle cells in submucosal layer are also positive for desmin.
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Immunocytochemistry/ Immunofluorescence - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)This image is courtesy of an anonymous Abreview

Unpurified ab32362 staining Desmin (green) in Mouse aorta smooth muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formalin and blocked with 10% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/150) for 1 hour at 22°C. An Alexa Fluor^® 488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Blue - nuclei.
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This product has been referenced in:

Akiyama T et al. Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing. Sci Rep 8:1189 (2018). Read more (PubMed: 29352121) »
Westendorp BF et al. Indian Hedgehog Suppresses a Stromal Cell-Driven Intestinal Immune Response. Cell Mol Gastroenterol Hepatol 5:67-82.e1 (2018). Flow Cyt ; Mouse . Read more (PubMed: 29276753) »

See all 56 Publications for this product

Publishing research using ab32362? Please let us know so that we can cite the reference in this datasheet.

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1-10 of 12 Abreviews

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Sample

Rat Tissue lysate - whole (gastrocnemius)

Gel Running Conditions

Reduced Denaturing

Loading amount

50 µg

Specification

gastrocnemius

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Florine Tissier

Verified customer

Submitted Feb 05 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Prostate)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate, pH 6

Permeabilization

Specification

Prostate

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 02 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Skeletal muscle)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA

Permeabilization

Specification

Skeletal muscle

Blocking step

PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Dec 14 2017

Application

Immunocytochemistry/ Immunofluorescence

Sample

Sheep Cell (Myoblast)

Permeabilization

Specification

Myoblast

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

1:1 Acetone:Methanol

Miss. Barbara Makela

Verified customer

Submitted Jun 22 2017

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Cat Tissue sections (heart)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate bufffer pH6.0

Permeabilization

Specification

heart

Blocking step

BSA as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C

Fixative

Formaldehyde

Prof. 清和尾崎

Verified customer

Submitted Jan 24 2017

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Skin)

Permeabilization

Specification

Skin

Blocking step

Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 24°C

Fixative

Acetone

Abcam user community

Verified customer

Submitted Oct 26 2016

Application

Immunocytochemistry/ Immunofluorescence

Blocking step

BSA/PBS as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C

Sample

Human Cell (smooth muscle cell line)

Specification

smooth muscle cell line

Permeabilization

Yes - Triton X-100 0.1%

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 31 2015

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Immunocytochemistry/ Immunofluorescence

Blocking step

Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C

Sample

Human Cell (Skeletal Muscle)

Specification

Skeletal Muscle

Permeabilization

Fixative

Methacarn

Abcam user community

Verified customer

Submitted Sep 26 2013

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing (12% Bis Tis Gel)

Sample

Human Tissue lysate - whole (Skeletal Muscle Cells)

Specification

Skeletal Muscle Cells

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Sep 26 2013

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (Aorta Smooth Muscle Cells)

Specification

Aorta Smooth Muscle Cells

Blocking step

Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C

Fixative

Formalin

Abcam user community

Verified customer

Submitted Sep 26 2013

1-10 of 12 Abreviews

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Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

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Western blot abreview for Anti-Desmin antibody [Y66] - Cytoskeleton Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody [Y66] - Cytoskeleton Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody [Y66] - Cytoskeleton Marker

Immunocytochemistry/ Immunofluorescence abreview for Anti-Desmin antibody [Y66] - Cytoskeleton Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody [Y66] - Cytoskeleton Marker

Immunohistochemistry (Frozen sections) abreview for Anti-Desmin antibody [Y66] - Cytoskeleton Marker

Immunocytochemistry/ Immunofluorescence abreview for Anti-Desmin antibody [Y66] - Cytoskeleton Marker

Immunocytochemistry/ Immunofluorescence abreview for Anti-Desmin antibody [Y66]

Western blot abreview for Anti-Desmin antibody [Y66]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Desmin antibody [Y66]