For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    desmin-antibody-y66-cytoskeleton-marker-ab32362.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class III Desmin
Share by email
RecombinantRabMAb

Recombinant Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

  • Datasheet
  • SDS
Reviews (12)Q&A (4)References (127)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
  • Immunocytochemistry - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
  • Western blot - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
  • Immunocytochemistry - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
  • Immunocytochemistry - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
  • Flow Cytometry (Intracellular) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
  • Immunocytochemistry - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
  • Western blot - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
  • Western blot - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
  • Western blot - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
  • Immunocytochemistry - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
  • Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y66] to Desmin - Cytoskeleton Marker
  • Suitable for: WB, IHC-P, Flow Cyt (Intra), ICC
  • Reacts with: Mouse, Rat, Guinea pig, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 APC Biotin Carrier Free HRP PE

You may also be interested in

Primary
Product image
Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] (ab133292)
Primary
Product image
Alexa Fluor® 488 Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab185033)
Primary
Product image
Alexa Fluor® 647 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192980)

View more associated products

Overview

  • Product name

    Anti-Desmin antibody [Y66] - Cytoskeleton Marker
    See all Desmin primary antibodies
  • Description

    Rabbit monoclonal [Y66] to Desmin - Cytoskeleton Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt (Intra), ICCmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Guinea pig, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Epitope

    ab32362 reacts with an epitope located in the C terminal region of desmin.
  • Positive control

    • WB: Human skeletal muscle, fetal heart and fetal muscle tissue lysates. Mouse and rat heart tissue lysates. Guinea pig heart and muscle tissue lysates; ICC: A673 and C2C12 cells, ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612); IHC-P: Human skeletal muscle, uterus and urinary bladder tissues; Flow Cyt (intra): C2C12 and HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    Y66
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class III
    • Desmin
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other
    • Cardiovascular
    • Heart
    • Cardiac arrhythmias

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab185033)
    • Alexa Fluor® 647 Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab195177)
    • HRP Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab195178)
    • Biotin Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab197603)
    • Alexa Fluor® 594 Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab203419)
    • Alexa Fluor® 555 Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab203422)
    • Anti-Desmin antibody [Y66] - Low endotoxin, Azide free (ab216616)
    • APC Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab224934)
    • PE Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab224935)
    • Anti-Desmin antibody [Y66] - BSA and Azide free (ab271829)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Mouse heart normal tissue lysate - total protein (ab30291)
    • Mouse heart tissue lysate - total protein (0 days) (ab7193)
    • Mouse heart tissue lysate - total protein (14 days) (ab7194)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/100000. Predicted molecular weight: 53 kDa.
IHC-P (4)
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt (Intra)
1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC
1/100 - 1/1000.
Notes
WB
1/100000. Predicted molecular weight: 53 kDa.
IHC-P
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt (Intra)
1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC
1/100 - 1/1000.
Application notes
Is unsuitable for IP.

Target

  • Function

    Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
  • Involvement in disease

    Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
    Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P17661 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1674 Human
    • Entrez Gene: 13346 Mouse
    • Entrez Gene: 64362 Rat
    • Omim: 125660 Human
    • SwissProt: P17661 Human
    • SwissProt: P31001 Mouse
    • SwissProt: P48675 Rat
    • Unigene: 594952 Human
    • Unigene: 6712 Mouse
    • Unigene: 39196 Rat
    see all
  • Alternative names

    • CMD1I antibody
    • CSM1 antibody
    • CSM2 antibody
    • DES antibody
    • DESM_HUMAN antibody
    • Desmin antibody
    • FLJ12025 antibody
    • FLJ39719 antibody
    • FLJ41013 antibody
    • FLJ41793 antibody
    • Intermediate filament protein antibody
    • OTTHUMP00000064865 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling Desmin with purified ab32362 at 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
    Immunocytochemistry - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

    Immunofluorescence staining of Desmin using ab32362 in ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), which were differentiated for 10 days post induction.


    The cells were fixed with 100% MeOH (5 min) and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab32362 at 0.02 µg/mL and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin, at 1/1000 dilution. Cells were then incubated with ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).


    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.


    The antibody ab32362 gave comparable results using 4% formaldehyde fixation (10 min).

  • Western blot - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
    Western blot - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
    All lanes : Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362) at 1/500000 dilution (purified)

    Lane 1 : Human skeletal muscle tissue lysate
    Lane 2 : Human fetal heart tissue lysate
    Lane 3 : Human fetal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunocytochemistry - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
    Immunocytochemistry - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)This image is courtesy of an anonymous Abreview

    Unpurified ab32362 staining Desmin (green) in Human skeletal muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methacarn and blocked with 10% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/150) for 12 hours. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Blue - DAPI-nuclei. Red - WGA. 40X objective.

    See Abreview

  • Immunocytochemistry - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
    Immunocytochemistry - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

    Immunocytochemistry/Immunofluorescence analysis of C2C12 (Mouse myoblasts myoblast) cells labeling Desmin with ab32362 at 1/500. Cells were fixed with 100% Methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. 

     

    ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 was used as counterstain antibody.

    Confocal image showing cytoplasmic staining on C2C12 cell line.

     

  • Flow Cytometry (Intracellular) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
    Flow Cytometry (Intracellular) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

    Intracellular Flow Cytometry analysis of C2C12 cells labelling Desmin with purified ab32362 at 1/70 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies. 

     

     

  • Immunocytochemistry - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
    Immunocytochemistry - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

    Immunocytochemistry/Immunofluorescence analysis of A673 cells labelling Desmin with purified ab32362 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/50) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)Cowan DB et al. Intracoronary Delivery of Mitochondria to the Ischemic Heart for Cardioprotection. PLoS One 11:e0160889 (2016). Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunofluorescent analysis of Human mitochondria injected rabbit hearts sections stained for Desmin (Green) using ab32362. MTCO2, the human-specific mitochondrial marker was stained in red, and the nuclei was stained using the DNA stain DAPI (blue).

  • Western blot - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
    Western blot - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
    All lanes : Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362) at 1/500000 dilution (purified)

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Rat heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
    Western blot - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
    All lanes : Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362) at 1/100000 dilution

    Lane 1 : Guinea pig heart tissue lysate
    Lane 2 : Guinea pig muscle tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
    Western blot - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)This image is courtesy of an anonymous Abreview
    All lanes : Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362) at 1/500 dilution (unpurified)

    All lanes : Human skeletal muscle whole tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : IRDye® 680-conjugated anti-rabbit at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 53 kDa


    Exposure time: 50 seconds

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

    Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of normal human uterus tissue labelling Desmin with unpurified ab32362.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

    Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of normal human urinary bladder tissue labelling Desmin with unpurified ab32362.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

    Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human skeletal muscle tissue labelling Desmin with unpurified ab32362.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

    Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of normal human brain tissue. Unpurified ab32362 shows negative staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

    Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of normal human tonsil tissue. Unpurified ab32362 shows negative staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
    Immunocytochemistry - Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)This image is courtesy of an anonymous Abreview

    Unpurified ab32362 staining Desmin (green) in Mouse aorta smooth muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formalin and blocked with 10% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/150) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Blue - nuclei.

    See Abreview

  • Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
    Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (127)

Publishing research using ab32362? Please let us know so that we can cite the reference in this datasheet.

ab32362 has been referenced in 127 publications.

  • Schwabl P  et al. The Non-Steroidal FXR Agonist Cilofexor Improves Portal Hypertension and Reduces Hepatic Fibrosis in a Rat NASH Model. Biomedicines 9:N/A (2021). PubMed: 33435509
  • Qian FY  et al. A novel recessive mutation affecting DNAJB6a causes myofibrillar myopathy. Acta Neuropathol Commun 9:23 (2021). PubMed: 33557929
  • Huang LL  et al. Integrin-Linked Kinase (ILK) Regulates Urinary Stem Cells Differentiation into Smooth Muscle via NF-?B Signal Pathway. Stem Cells Int 2021:6633111 (2021). PubMed: 33854551
  • Sukhacheva TV  et al. Morpho-functional changes of cardiac telocytes in isolated atrial amyloidosis in patients with atrial fibrillation. Sci Rep 11:3563 (2021). PubMed: 33574429
  • Bilhar APM  et al. Long-term effects of muscle-derived stem cell therapy on the regeneration of the urethra of female rats. Int Urogynecol J N/A:N/A (2021). PubMed: 33655365
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review

Filter by Application

Filter by Species

Filter by Ratings

1-10 of 12 Abreviews

Western blot abreview for Anti-Desmin antibody [Y66] - Cytoskeleton Marker

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (gastrocnemius)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
gastrocnemius
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Dr. Florine Tissier

Verified customer

Submitted Feb 05 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody [Y66] - Cytoskeleton Marker

Below Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Prostate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate, pH 6
Permeabilization
No
Specification
Prostate
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jan 02 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody [Y66] - Cytoskeleton Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Skeletal muscle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
Permeabilization
No
Specification
Skeletal muscle
Blocking step
PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Dec 14 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-Desmin antibody [Y66] - Cytoskeleton Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Sheep Cell (Myoblast)
Permeabilization
No
Specification
Myoblast
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
1:1 Acetone:Methanol
Read More

MISS. Barbara Makela

Verified customer

Submitted Jun 22 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody [Y66] - Cytoskeleton Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate bufffer pH6.0
Permeabilization
No
Specification
heart
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Prof. 清和 尾崎

Verified customer

Submitted Jan 24 2017

Immunohistochemistry (Frozen sections) abreview for Anti-Desmin antibody [Y66] - Cytoskeleton Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Skin)
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

Submitted Oct 26 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-Desmin antibody [Y66] - Cytoskeleton Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA/PBS as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Human Cell (smooth muscle cell line)
Specification
smooth muscle cell line
Permeabilization
Yes - Triton X-100 0.1%
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Mar 31 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Desmin antibody [Y66]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
Sample
Human Cell (Skeletal Muscle)
Specification
Skeletal Muscle
Permeabilization
No
Fixative
Methacarn
Read More

Abcam user community

Verified customer

Submitted Sep 26 2013

Western blot abreview for Anti-Desmin antibody [Y66]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% Bis Tis Gel)
Sample
Human Tissue lysate - whole (Skeletal Muscle Cells)
Specification
Skeletal Muscle Cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted Sep 26 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-Desmin antibody [Y66]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Aorta Smooth Muscle Cells)
Specification
Aorta Smooth Muscle Cells
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formalin
Read More

Abcam user community

Verified customer

Submitted Sep 26 2013

1-10 of 12 Abreviews

  •  Previous
  • 1
  • 2
  • Next 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.