Product nameAnti-Desmin antibody [Y66] - Cytoskeleton Marker (Allophycocyanin)
See all Desmin primary antibodies
DescriptionRabbit monoclonal [Y66] to Desmin - Cytoskeleton Marker (Allophycocyanin)
ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Guinea pig, Human
Synthetic peptide corresponding to Human Desmin aa 400 to the C-terminus (C terminal).
Epitopeab224934 reacts with an epitope located in the C terminal region of desmin.
- Flow Cyt: SV40LT-SMC cells
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-Desmin antibody [Y66] - Cytoskeleton Marker (Alexa Fluor® 488) (ab185033)
- Anti-Desmin antibody [Y66] - Cytoskeleton Marker (Alexa Fluor® 647) (ab195177)
- Anti-Desmin antibody [Y66] - Cytoskeleton Marker (HRP) (ab195178)
- Anti-Desmin antibody [Y66] - Cytoskeleton Marker (Biotin) (ab197603)
- Anti-Desmin antibody [Y66] - Cytoskeleton Marker (Alexa Fluor® 568) (ab202503)
- Anti-Desmin antibody [Y66] - Cytoskeleton Marker (Alexa Fluor® 594) (ab203419)
- Anti-Desmin antibody [Y66] - Cytoskeleton Marker (Alexa Fluor® 555) (ab203422)
- Anti-Desmin antibody [Y66] - Low endotoxin, Azide free (ab216616)
- Anti-Desmin antibody [Y66] - Cytoskeleton Marker (Phycoerythrin) (ab224935)
- Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
Our Abpromise guarantee covers the use of ab224934 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The cellular localisation of this product has been verified in ICC/IF.
FunctionDesmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
Involvement in diseaseDefects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin.
Sequence similaritiesBelongs to the intermediate filament family.
- Information by UniProt
- CMD1I antibody
- CSM1 antibody
- CSM2 antibody
Overlay histogram showing SV40LT-SMC cells stained with ab224934 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab224934, 1/5000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.
This antibody gave a positive signal in SV40LT-SMC cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
ab224934 has not yet been referenced specifically in any publications.