Recombinant Anti-Desmocollin 1 antibody [EPR8220] (ab150382)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8220] to Desmocollin 1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Desmocollin 1 antibody [EPR8220]
See all Desmocollin 1 primary antibodies -
Description
Rabbit monoclonal [EPR8220] to Desmocollin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Desmocollin 1 aa 850 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q08554 -
Positive control
- WB: A375 and A431 whole cell lysates. Human skin tissue lysate. Mouse brain, skin, and kidney tissue lysates. Rat brain and kidney tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8220 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150382 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000. Predicted molecular weight: 100 kDa.
For unpurified use at 1/1000 - 1/10000. |
Notes |
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WB
1/10000. Predicted molecular weight: 100 kDa. For unpurified use at 1/1000 - 1/10000. |
Target
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Relevance
Desmocollin 1 is a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. It may contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms. Desmocollin 1 is linked to the keratinization of epithelial tissues. -
Cellular localization
Cell membrane; Single-pass type I membrane protein. Cell junction; desmosome. -
Database links
- Entrez Gene: 1823 Human
- Entrez Gene: 13505 Mouse
- Entrez Gene: 291759 Rat
- Omim: 125643 Human
- SwissProt: Q08554 Human
- SwissProt: P55849 Mouse
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Alternative names
- Cadherin family member 1 antibody
- CDHF1 antibody
- Desmocollin1 antibody
see all
Images
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All lanes : Anti-Desmocollin 1 antibody [EPR8220] (ab150382) at 1/10000 dilution (purified)
Lane 1 : A375 (Human malignant melanoma epithelial cell) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 3 : Human skin lysate
Lane 4 : Mouse brain lysate
Lane 5 : Mouse spleen lysate
Lane 6 : Mouse kidney lysate
Lane 7 : Rat brain lysate
Lane 8 : Rat kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDaWe are unsure about the extra bands. They might be the deglycosylated forms as described in PMID: 25918164.
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All lanes : Anti-Desmocollin 1 antibody [EPR8220] (ab150382) at 1/500000 dilution
Lane 1 : Empty vector transfected 293T (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : 293T cells transfected with myc-his tagged full length Desmocollin-1 expression vector, whole cell lysate
Lane 3 : 293T cells transfected with myc-his tagged full length Desmocollin-2 expression vector, whole cell lysate
Lane 4 : 293T cells transfected with myc-his tagged full length Desmocollin-3 expression vector, whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 7 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab150382 has been referenced in 2 publications.
- Shuck SC et al. MLS128 antibody-induced suppression of colon cancer cell growth is mediated by a desmocollin and a 110 kDa glycoprotein. Biosci Trends 13:216-224 (2019). PubMed: 31168022
- Civciristov S et al. Ligand-dependent spatiotemporal signaling profiles of the µ-opioid receptor are controlled by distinct protein-interaction networks. J Biol Chem 294:16198-16213 (2019). PubMed: 31515267