Recombinant Anti-Desmocollin 3 antibody [EPR7486] (ab126768)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7486] to Desmocollin 3
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Desmocollin 3 antibody [EPR7486]
See all Desmocollin 3 primary antibodies -
Description
Rabbit monoclonal [EPR7486] to Desmocollin 3 -
Host species
Rabbit -
Specificity
This antibody cross-reacts weakly with Desmocollin 2.
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Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- A431, Human skin, and Human espophagus lysates; and A431 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.40 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7486 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126768 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 100 kDa.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Predicted molecular weight: 100 kDa. |
Target
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Function
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms. -
Tissue specificity
Epidermis, buccal mucosa, esophagus and cervix. -
Involvement in disease
Defects in DSC3 are the cause of hypotrichosis and recurrent skin vesicles (HRSV) [MIM:613102]. A disorder characterized by hypotrichosis and the appearance of recurrent skin vesicle formation. Affected individuals show sparse and fragile hair on scalp, as well as absent eyebrows and eyelashes. Vesicles filled with thin, watery fluid are observed on the scalp and skin of most of the boby. Mucosal vesicles are absent. -
Sequence similarities
Contains 5 cadherin domains. -
Domain
Calcium may be bound by the cadherin-like repeats. -
Cellular localization
Cell membrane. Cell junction > desmosome. - Information by UniProt
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Database links
- Entrez Gene: 1825 Human
- Entrez Gene: 13507 Mouse
- Entrez Gene: 307563 Rat
- Omim: 600271 Human
- SwissProt: Q14574 Human
- SwissProt: P55850 Mouse
- Unigene: 41690 Human
- Unigene: 89935 Mouse
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Alternative names
- Cadherin family member 3 antibody
- CDHF3 antibody
- desmocollin 3a/3b antibody
see all
Images
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All lanes : Anti-Desmocollin 3 antibody [EPR7486] (ab126768) at 1/200000 dilution
Lane 1 : Empty vector transfected 293T (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : 293T cells transfected with myc-his tagged full length Desmocollin-1 expression vector, whole cell lysate
Lane 3 : 293T cells transfected with myc-his tagged full length Desmocollin-2 expression vector, whole cell lysate
Lane 4 : 293T cells transfected with myc-his tagged full length Desmocollin-3 expression vector, whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 40 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
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ab126768 at 1/10 dilution staining Desmocollin 3 in permeabilized A431 cells by intracellular flow cytometry (red) compared with a rabbit IgG negative control (green).
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All lanes : Anti-Desmocollin 3 antibody [EPR7486] (ab126768) at 1/10000 dilution
Lane 1 : A431 cell lysate
Lane 2 : Human skin lysate
Lane 3 : Human espophagus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 100 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab126768 has been referenced in 3 publications.
- Wang J et al. The proliferative and anti-apoptosis functions of KGF/KGFR contributes to bronchial epithelial repair in asthma. Pulm Pharmacol Ther 63:101931 (2020). PubMed: 32818548
- Bir F et al. New immunohistochemical markers in the differential diagnosisof nonsmall cell lung carcinoma. Turk J Med Sci 46:1854-1861 (2016). PubMed: 28081338
- Passantino L et al. Sodium metavanadate exhibits carcinogenic tendencies in vitro in immortalized human bronchial epithelial cells. Metallomics 5:1357-67 (2013). WB ; Human . PubMed: 23963610