• Product name
    Anti-Desmoglein 1 antibody [27B2]
    See all Desmoglein 1 primary antibodies
  • Description
    Mouse monoclonal [27B2] to Desmoglein 1
  • Host species
  • Specificity
    Ab12077 reacts with Desmoglein 1. Does not cross-react with Desmoglein 2 or Desmoglein 3.
  • Tested applications
    Suitable for: IHC-P, IP, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Unfortunately, this information is considered to be commercially sensitive

  • Positive control
    • IHC-Fr: Human skin



Our Abpromise guarantee covers the use of ab12077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/250.

Tissue sections should be fixed with cold methanol prior to staining, for better results.


  • Function
    Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
  • Tissue specificity
    Epidermis, tongue, tonsil and esophagus.
  • Involvement in disease
    Defects in DSG1 are the cause of palmoplantar keratoderma striate type 1 (SPPK1) [MIM:148700]; also known as keratosis palmoplantaris striata I. SPPK1 is a dermatoligical disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger.
  • Sequence similarities
    Contains 4 cadherin domains.
  • Domain
    Calcium may be bound by the cadherin-like repeats.
  • Cellular localization
    Cell membrane. Cell junction > desmosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cadherin family member 4 antibody
    • CDHF4 antibody
    • Desmoglein-1 antibody
    • Desmosomal glycoprotein 1 antibody
    • DG1 antibody
    • DGI antibody
    • DSG antibody
    • DSG1 antibody
    • DSG1_HUMAN antibody
    • EPKHE antibody
    • EPKHIA antibody
    • Pemphigus foliaceus antigen antibody
    • PPKS1 antibody
    • SPPK1 antibody
    see all


  • IHC image of ab12077 staining in 100% methanol fixed frozen tissue section of human skin.

    Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with ab12077 (1μg/ml dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647)) and DAPI for 1 hour at room temperature.

    The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab12077. 

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.


  • ab12077 staining Desmoglein 1 in human skin tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in methanol, permeabilized using 0.4% Triton X-100, blocked with 10% serum for 30 minutes at 25°C, then incubated with ab12077 at 5µg/ml for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated goat anti-mouse polyclonal (highly adsorbed), used at a 1/1000 dilution.

    See Abreview


This product has been referenced in:
  • Tsutsumi M  et al. Mechanical-stimulation-evoked calcium waves in proliferating and differentiated human keratinocytes. Cell Tissue Res 338:99-106 (2009). IHC-Fr ; Human . Read more (PubMed: 19657674) »
  • Zhang X & Tseng H Basonuclin-null mutation impairs homeostasis and wound repair in mouse corneal epithelium. PLoS ONE 2:e1087 (2007). IHC-Fr ; Mouse . Read more (PubMed: 17971852) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Tonsil epithelium)
Tonsil epithelium
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Tris pH=7.4/ 1mMEDTA

Abcam user community

Verified customer

Submitted Jan 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Frozen sections)
Human Tissue sections (skin)
Yes - 0.4% TritonX-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 08 2011


I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab10277. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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Thank you for your enquiry. According to the NCBI-protein site aa 664-763 are located in the cytoplasmic region of the protein. The cytoplasmic staining is therefore correct. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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