Key features and details
- Mouse monoclonal [27B2] to Desmoglein 1/DSG1
- Suitable for: IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG1
Product nameAnti-Desmoglein 1/DSG1 antibody [27B2]
See all Desmoglein 1/DSG1 primary antibodies
DescriptionMouse monoclonal [27B2] to Desmoglein 1/DSG1
Tested Applications & Species
Application Species IHC-FrHuman
The details of the immunogen for this antibody are not available.
- IHC-Fr: Human normal skin.
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact firstname.lastname@example.org.
This product was previously labelled as Desmoglein 1
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Light chain typekappa
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Use a concentration of 1 µg/ml.
Use a concentration of 1 µg/ml.
FunctionComponent of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
Tissue specificityEpidermis, tongue, tonsil and esophagus.
Involvement in diseaseDefects in DSG1 are the cause of palmoplantar keratoderma striate type 1 (SPPK1) [MIM:148700]; also known as keratosis palmoplantaris striata I. SPPK1 is a dermatoligical disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger.
Sequence similaritiesContains 4 cadherin domains.
DomainCalcium may be bound by the cadherin-like repeats.
Cellular localizationCell membrane. Cell junction > desmosome.
- Information by UniProt
- Cadherin family member 4 antibody
- CDHF4 antibody
- Desmoglein-1 antibody
IHC image of ab12077 staining in 100% methanol fixed frozen tissue section of human skin.
Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with ab12077 (1μg/ml dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647)) and DAPI for 1 hour at room temperature.
The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab12077.
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
ab12077 staining Desmoglein 1/DSG1 in human skin tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in methanol, permeabilized using 0.4% Triton X-100, blocked with 10% serum for 30 minutes at 25°C, then incubated with ab12077 at 5µg/ml for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated goat anti-mouse polyclonal (highly adsorbed), used at a 1/1000 dilution.
ab12077 has been referenced in 4 publications.
- Yuan J et al. SHROOM2 inhibits tumor metastasis through RhoA-ROCK pathway-dependent and -independent mechanisms in nasopharyngeal carcinoma. Cell Death Dis 10:58 (2019). PubMed: 30683844
- Tsutsumi M et al. Mechanical-stimulation-evoked calcium waves in proliferating and differentiated human keratinocytes. Cell Tissue Res 338:99-106 (2009). IHC-Fr ; Human . PubMed: 19657674
- Zhang X & Tseng H Basonuclin-null mutation impairs homeostasis and wound repair in mouse corneal epithelium. PLoS ONE 2:e1087 (2007). IHC-Fr ; Mouse . PubMed: 17971852
- Wu H et al. Desmoglein isotype expression in the hair follicle and its cysts correlates with type of keratinization and degree of differentiation. J Invest Dermatol 120:1052-7 (2003). PubMed: 12787134