Anti-Desmoglein 1/DSG1 antibody [27B2] (ab12077)
![Immunohistochemistry (Frozen sections) - Anti-Desmoglein 1/DSG1 antibody [27B2] (ab12077)](https://www.abcam.com/ps/products/12/ab12077/Images/ab12077-336311-anti-desmoglein-1-antibody-27b2-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-Desmoglein 1/DSG1 antibody [27B2] (ab12077)")
Key features and details
- Mouse monoclonal [27B2] to Desmoglein 1/DSG1
- Suitable for: IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-Desmoglein 1/DSG1 antibody [27B2]
See all Desmoglein 1/DSG1 primary antibodies -
Description
Mouse monoclonal [27B2] to Desmoglein 1/DSG1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr Human
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Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- IHC-Fr: Human normal skin.
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General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This product was previously labelled as Desmoglein 1
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
27B2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-Fr |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | (1) |
Use a concentration of 1 µg/ml.
|
| Notes |
|---|
|
IHC-Fr
Use a concentration of 1 µg/ml. |
Target
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Function
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. -
Tissue specificity
Epidermis, tongue, tonsil and esophagus. -
Involvement in disease
Defects in DSG1 are the cause of palmoplantar keratoderma striate type 1 (SPPK1) [MIM:148700]; also known as keratosis palmoplantaris striata I. SPPK1 is a dermatoligical disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger. -
Sequence similarities
Contains 4 cadherin domains. -
Domain
Calcium may be bound by the cadherin-like repeats. -
Cellular localization
Cell membrane. Cell junction > desmosome. - Information by UniProt
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Database links
- Entrez Gene: 1828 Human
- Entrez Gene: 13510 Mouse
- Omim: 125670 Human
- SwissProt: Q02413 Human
- SwissProt: Q61495 Mouse
- Unigene: 2633 Human
- Unigene: 37953 Mouse
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Alternative names
- Cadherin family member 4 antibody
- CDHF4 antibody
- Desmoglein-1 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-Desmoglein 1/DSG1 antibody [27B2] (ab12077)
IHC image of ab12077 staining in 100% methanol fixed frozen tissue section of human skin.
Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with ab12077 (1μg/ml dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647)) and DAPI for 1 hour at room temperature.
The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab12077.
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
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![Immunohistochemistry (Frozen sections) - Anti-Desmoglein 1/DSG1 antibody [27B2] (ab12077)](https://www.abcam.com/ps/products/12/ab12077/Images/ab12077-19418-anti-desmoglein-1-antibody-27b2-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Desmoglein 1/DSG1 antibody [27B2] (ab12077)This image is courtesy of an anonymous Abreview.ab12077 staining Desmoglein 1/DSG1 in human skin tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in methanol, permeabilized using 0.4% Triton X-100, blocked with 10% serum for 30 minutes at 25°C, then incubated with ab12077 at 5µg/ml for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated goat anti-mouse polyclonal (highly adsorbed), used at a 1/1000 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab12077 has been referenced in 4 publications.
- Yuan J et al. SHROOM2 inhibits tumor metastasis through RhoA-ROCK pathway-dependent and -independent mechanisms in nasopharyngeal carcinoma. Cell Death Dis 10:58 (2019). PubMed: 30683844
- Tsutsumi M et al. Mechanical-stimulation-evoked calcium waves in proliferating and differentiated human keratinocytes. Cell Tissue Res 338:99-106 (2009). IHC-Fr ; Human . PubMed: 19657674
- Zhang X & Tseng H Basonuclin-null mutation impairs homeostasis and wound repair in mouse corneal epithelium. PLoS ONE 2:e1087 (2007). IHC-Fr ; Mouse . PubMed: 17971852
- Wu H et al. Desmoglein isotype expression in the hair follicle and its cysts correlates with type of keratinization and degree of differentiation. J Invest Dermatol 120:1052-7 (2003). PubMed: 12787134