Recombinant
RabMAb

Recombinant Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490)

Overview

  • Product name

    Anti-Desmoglein 1/DSG1 antibody [EPR20382]
    See all Desmoglein 1/DSG1 primary antibodies
  • Description

    Rabbit monoclonal [EPR20382] to Desmoglein 1/DSG1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Desmoglein 1/DSG1 aa 250-500. The exact sequence is proprietary.
    Database link: Q02413

  • Positive control

    • WB: Human skin tissue lysate. IHC-P: Human esophagus and skin tissue.
  • General notes

     This product was previously labelled as Desmoglein 1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab209490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 114 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
  • Tissue specificity

    Epidermis, tongue, tonsil and esophagus.
  • Involvement in disease

    Defects in DSG1 are the cause of palmoplantar keratoderma striate type 1 (SPPK1) [MIM:148700]; also known as keratosis palmoplantaris striata I. SPPK1 is a dermatoligical disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger.
  • Sequence similarities

    Contains 4 cadherin domains.
  • Domain

    Calcium may be bound by the cadherin-like repeats.
  • Cellular localization

    Cell membrane. Cell junction > desmosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cadherin family member 4 antibody
    • CDHF4 antibody
    • Desmoglein-1 antibody
    • Desmosomal glycoprotein 1 antibody
    • DG1 antibody
    • DGI antibody
    • DSG antibody
    • DSG1 antibody
    • DSG1_HUMAN antibody
    • EPKHE antibody
    • EPKHIA antibody
    • Pemphigus foliaceus antigen antibody
    • PPKS1 antibody
    • SPPK1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Desmoglein 1/DSG1 with ab209490 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on human skin (PMID 15606502) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

    Heat-mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).

  • Immunohistochemical analysis of paraffin-embedded human esophagus tissue labeling Desmoglein 1/DSG1 with ab209490 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Membranous staining on human esophagus (PMID: 24220297) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

    Heat-mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).

  • Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490) at 1/1000 dilution + Human skin lysate at 10 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 114 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?


    Exposure time: 26 seconds


    Blocking and dilution buffer: 5% NFDM/TBST 

References

ab209490 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab209490.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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