Recombinant
RabMAb

Recombinant Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798)

Overview

  • Product name

    Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)]
    See all Desmoglein 1/DSG1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6766(B)] to Desmoglein 1/DSG1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Desmoglein 1/DSG1 aa 1000 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q02413

  • Positive control

    • Human skin lysate, Human skin tissue
  • General notes

     

     

     This product was previously labelled as Desmoglein 1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 114 kDa.
IHC-P 1/250 - 1/500. antigen retrieval is recommended.
  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function

      Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
    • Tissue specificity

      Epidermis, tongue, tonsil and esophagus.
    • Involvement in disease

      Defects in DSG1 are the cause of palmoplantar keratoderma striate type 1 (SPPK1) [MIM:148700]; also known as keratosis palmoplantaris striata I. SPPK1 is a dermatoligical disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger.
    • Sequence similarities

      Contains 4 cadherin domains.
    • Domain

      Calcium may be bound by the cadherin-like repeats.
    • Cellular localization

      Cell membrane. Cell junction > desmosome.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cadherin family member 4 antibody
      • CDHF4 antibody
      • Desmoglein-1 antibody
      • Desmosomal glycoprotein 1 antibody
      • DG1 antibody
      • DGI antibody
      • DSG antibody
      • DSG1 antibody
      • DSG1_HUMAN antibody
      • EPKHE antibody
      • EPKHIA antibody
      • Pemphigus foliaceus antigen antibody
      • PPKS1 antibody
      • SPPK1 antibody
      see all

    Images

    • Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798) at 1/1000 dilution + Human skin lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 114 kDa

    • ab124798, at a 1/250 dilution, staining Desmoglein 1/DSG1 in paraffin embedded Human skin tissue by Immunohistochemistry.

    References

    This product has been referenced in:

    • Murphy S  et al. Proteomic analysis of the sarcolemma-enriched fraction from dystrophic mdx-4cv skeletal muscle. J Proteomics 191:212-227 (2019). Read more (PubMed: 29408692) »
    • Sumitomo T  et al. Streptococcal Cysteine Protease-Mediated Cleavage of Desmogleins Is Involved in the Pathogenesis of Cutaneous Infection. Front Cell Infect Microbiol 8:10 (2018). IHC-P . Read more (PubMed: 29416987) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (skin)
    Specification
    skin
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Acetone

    Abcam user community

    Verified customer

    Submitted Mar 24 2015

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (epidermis)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Permeabilization
    No
    Specification
    epidermis
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted May 29 2013

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Epidermis)
    Permeabilization
    No
    Specification
    Epidermis
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Methanol

    Abcam user community

    Verified customer

    Submitted May 29 2013

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (epidermis)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    epidermis
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted May 29 2013

    Answer

    Thank you for contacting us. Although we do not have DSG 1 and 3 antibodies that have been tested for reactivity with horse or even in all the applications you anticipate using, we do have at least one DSG1 antibody that I think will be useful.

    The catalogue number is ab124798:

    Click here (or use the following: https://www.abcam.com/index.html?datasheet=124798).

    The immunogen sequence is 93% conserved in the horse sequence you sent (14 of 15 amino acids are identical), so it is likely that ab124798 will cross-react with the DSG1-like horse protein. The antibody has not been tested in IHC on frozen tissue sections, only paraffin-embedded tissue. It has not been tested by flow cytometry, but does work in immunocytochemistry, and is likely to work for flow cytometry as well.

    For DSG3, the best candidate is ab14416, a mouse monoclonal raised against the extracellular portion of human DSG3. This region is 84% conserved in the horse sequence you sent. We do not know where the epitope that ab14416 recognizes falls within the extracellular region, so it is impossible to say if the epitope (generally consisting of a 6 - 8 amino acid sequence) is more or less than 84% identical to the homologous horse sequence. Again, the antibody has not been tested in all the applications you specified.

    Click here (or use the following: https://www.abcam.com/index.html?datasheet=14416).

    I hope this helps. Please let me know if you have any questions.

    Read More

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