Anti-Desmoglein 2/DSG2 antibody (ab96761)
Key features and details
- Rabbit polyclonal to Desmoglein 2/DSG2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Desmoglein 2/DSG2 antibody
See all Desmoglein 2/DSG2 primary antibodies -
Description
Rabbit polyclonal to Desmoglein 2/DSG2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Desmoglein 2/DSG2 aa 1-176.
Database link: NP_001934 -
Positive control
- WB: 293T, H1299 and MOLT4 whole cell lysates; HepG2 cell lysate; HeLa cell lysate. IHC-P: DU145 xenograft ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96761 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 122 kDa.
Blocking in 5% non-fat milk in TBST for 60 minutes at room temperature. |
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IHC-P |
1/100 - 1/500.
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Notes |
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ICC/IF
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 122 kDa. Blocking in 5% non-fat milk in TBST for 60 minutes at room temperature. |
IHC-P
1/100 - 1/500. |
Target
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Function
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. -
Tissue specificity
All of the tissues tested and carcinomas. -
Involvement in disease
Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10) [MIM:610193]; also known as arrhythmogenic right ventricular cardiomyopathy 10 (ARVC10). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
Genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB) [MIM:612877]. A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
Sequence similarities
Contains 4 cadherin domains. -
Domain
Calcium may be bound by the cadherin-like repeats. -
Cellular localization
Cell membrane. Cell junction > desmosome. - Information by UniProt
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Database links
- Entrez Gene: 1829 Human
- Entrez Gene: 13511 Mouse
- Omim: 125671 Human
- SwissProt: Q14126 Human
- SwissProt: O55111 Mouse
- Unigene: 412597 Human
- Unigene: 345891 Mouse
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Alternative names
- ARVC 10 antibody
- ARVC10 antibody
- ARVD 10 antibody
see all
Images
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Paraffin-embedded mouse skin labeling Desmoglein 2/DSG2 using ab96761 at 1/500 dilution in immunohistochemical analysis.
Antigen retrieval: EDTA-based (pH 8.0) 15 minutes.
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Paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells labeling Desmoglein 2/DSG2 using ab96761 at 1/500 dilution (green) in ICC/IF.
The nuclear counter stain is Hoechst 33342 (blue).
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Anti-Desmoglein 2/DSG2 antibody (ab96761) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 30 µg
Secondary
Anti-Rabbit IgG HRP
Predicted band size: 122 kDa -
All lanes : Anti-Desmoglein 2/DSG2 antibody (ab96761) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 122 kDa
7.5% SDS Page -
Immunohistochemical analysis of Desmoglein 2/DSG2 in paraffin embedded DU145 (Human) xenograft, using ab96761 at a 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab96761 has not yet been referenced specifically in any publications.