• Product name

    Anti-Desmoglein 2/DSG2 antibody [6D8]
    See all Desmoglein 2/DSG2 primary antibodies
  • Description

    Mouse monoclonal [6D8] to Desmoglein 2/DSG2
  • Host species

  • Specificity

    This antibody does not cross-react with Desmoglein 1 or Desmoglein 3. The epitope is within the extracellular domain of Desmoglein 2/DSG2. The clone number has been updated from (3G132) to (6D8) both clone numbers name the same antibody clone.

  • Tested applications

    Suitable for: IP, WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Desmoglein 2/DSG2.

  • General notes

    For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.


     This product was previously labelled as Desmoglein 2




Our Abpromise guarantee covers the use of ab14415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IF: Use at a concentration of 20 - 50 µg/ml.
    IHC-P: Used at a dilution of 1/10 for 30 min on human bladder cancer (see Abreview).
    IP: Use at a concentration of 5 - 10 µg/ml.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 165 kDa (predicted molecular weight: 122 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
    • Tissue specificity

      All of the tissues tested and carcinomas.
    • Involvement in disease

      Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10) [MIM:610193]; also known as arrhythmogenic right ventricular cardiomyopathy 10 (ARVC10). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
      Genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB) [MIM:612877]. A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    • Sequence similarities

      Contains 4 cadherin domains.
    • Domain

      Calcium may be bound by the cadherin-like repeats.
    • Cellular localization

      Cell membrane. Cell junction > desmosome.
    • Information by UniProt
    • Database links

    • Alternative names

      • ARVC 10 antibody
      • ARVC10 antibody
      • ARVD 10 antibody
      • ARVD10 antibody
      • Cadherin family member 5 antibody
      • CDHF 5 antibody
      • CDHF5 antibody
      • CMD1BB antibody
      • Desmoglein 2 antibody
      • Desmoglein-2 antibody
      • Desmoglein2 antibody
      • DSG 2 antibody
      • DSG2 antibody
      • DSG2_HUMAN antibody
      • HDGC antibody
      • HDGC included antibody
      • Human Desmoglein colon antibody
      • MGC117034 antibody
      • MGC117036 antibody
      • MGC117037 antibody
      see all


    • ab14415 at a 1/10 dilution staining human urothelium (bladder cancer) by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The antibody was incubated with the tissue for 30 minutes following heat mediated antigen retrieval and paraformaldehyde fixation.

      See Abreview

    • All lanes : Anti-Desmoglein 2/DSG2 antibody [6D8] (ab14415) at 3 µg/ml

      Lane 1 : U2OS (Hu) whole cell lysate
      Lane 2 : 293T (Hu) whole cell lysate
      Lane 3 : Cos-7 (AGMk) whole cell lysate
      Lane 4 : CHO (Hm) whole cell lysate
      Lane 5 : PC12 (Rat) whole cell lysate
      Lane 6 : 3T3 (Ms) whole cell lysate

      Lysates/proteins at 30 µg per lane.

      All lanes : HRP conjugated goat anti-mouse antibody

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 122 kDa
      Observed band size: 125 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.

      Exposure time: 3 minutes

      The blot was blocked with 5% milk for 1 hour at 21°C and incubated with the primary antibody for 12 hours at 4°C.

      The antibody detects a species of the predicted mobility (125 kDa) in Human cell lines U2OS (1) and 293T (2). No signal was observed in extracts from other species, suggesting that this antibody does not cross-react with AGMk, Hm, Ms or Rat.

      See Abreview


    This product has been referenced in:

    • Ebert LM  et al. A non-canonical role for desmoglein-2 in endothelial cells: implications for neoangiogenesis. Angiogenesis 19:463-86 (2016). IHC-P ; Mouse . Read more (PubMed: 27338829) »
    • Galoian K  et al. Restoration of desmosomal junction protein expression and inhibition of H3K9-specific histone demethylase activity by cytostatic proline-rich polypeptide-1 leads to suppression of tumorigenic potential in human chondrosarcoma cells. Mol Clin Oncol 3:171-178 (2015). Human . Read more (PubMed: 25469290) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    1-9 of 9 Abreviews or Q&A


    The epitope for ab14415 is located on the extracellular domain.

    With A431 cell lysate, this antibody detects a band at ˜165 kDa (represents apparent MW with post-translational modification; amino acid backbone has a calculated MW of ˜122 kDa.
    The abreview below suggests that the antibody is capable of detecting some Desmoglein 2 cleavage products:

    Read More
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Human Tissue sections (salivary gland)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Tris pH=7.4 / 1mM EDTA
    salivary gland

    Abcam user community

    Verified customer

    Submitted Jan 02 2013


    Thank you for your reply.

    I can confirm that this antibody does not cross-react with DSG1 or DSG3. Based on the information available on the other DSG2 antibodies, this is the only one I can confirm has been tested for such cross-reactivity.

    I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

    Read More


    Thank you for contacting Abcam regarding ab14415.

    I have run a sequence alignment of the human protein with the mouse sequence and found overall 76% identity. The specific epitope has not been mapped, thus it is difficult to determine if the antibody will cross react. Alignment with the Rhesus macaque sequence revealed 96% identity, thus there is a better chance of the antibody cross-reacting.

    If you are interested in testing this antibody, I would be happy to offer a testing discount in which you purchase this antibody, test it in your mouse or primate samples, and submit an Abreview, and you will receive a free primary antibody on a future order. Please let me know if you are interested and I would be happy to provide you with a discount code.

    I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

    Read More


    Vielen Dank für Ihre Geduld. Ich kann Ihnen jetzt auch bestätigen, dass der ab14415 ein Epitop auf dem extrazellulären Part des Desmoglein 2 erkennt. Auch der ab96761 erkennt das extrazelluläre Desmoglein 2, da der Antikörper gegen ein rekombinantes Fragment mit den Aminosäuren 1-166 des humanen Desmoglein 2 gemacht wurde. Diese Aminosäuren sind im extrazellulären Bereich dieses Proteins. http://www.uniprot.org/uniprot/Q14126?KeepThis=true& Ich hoffe diese Informationen sind Ihnen hilfreich. Bitte zögern Sie nicht uns wieder zu kontaktieren, sollten Sie weitere Fragen haben.

    Read More


    Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten. Ich habe das Labor angefragt, ob etwas bekannt ist im Bezug für das Epitops des ab14415 und des ab96761. Ich werde Sie wieder kontaktieren sobald ich eine Antwort bekommen haben. Der ab85632 erkennt eine Domäne die im extrazellulären Bereich des Proteins ist, ist jedoch unseres Wissens nach noch nicht in ICC getestet worden. Wie auch am Telefon besprochen, kann ich Ihnen momentan ein Testangebot machen, wenn Sie den ab85632 in Immunozytochemie (ICC) testen möchten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von ab85632 abdecken, und gegen eine erneute Bestellung eines weiteren primären Antikörper von uns verrechnet werden. Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten: 1.) Bestätigen Sie mir bitte, dass Sie ab85632 in ICC testen möchten. 2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes. 3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax 4.) Testen Sie den Antikörper in ICC . 5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes" Unter der folgenden URL können Sie mehr über unser Abreview System erfahren: https://www.abcam.com/abreviews 6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können einen anderen primären Antikörpers zu dem gleichen Preis wie ab85632 bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer von ab85632 bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss. Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist. Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen: https://www.abcam.com/collaborationdiscount Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

    Read More
    Human Cell lysate - whole cell (293T)
    Total protein in input
    250 µg
    Immuno-precipitation step
    Protein G

    Abcam user community

    Verified customer

    Submitted Dec 03 2007

    Western blot
    Human Cell lysate - whole cell (U2OS and 293T cell line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    U2OS and 293T cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

    Abcam user community

    Verified customer

    Submitted Nov 02 2007

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Human Tissue sections (Bladder cancer , normal urothelium)
    Bladder cancer , normal urothelium
    Antigen retrieval step
    Heat mediated

    Mr. Rudolf Jung

    Verified customer

    Submitted Aug 07 2006

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