Product nameAnti-Desmoglein 2/DSG2 antibody
See all Desmoglein 2/DSG2 primary antibodies
DescriptionRabbit polyclonal to Desmoglein 2/DSG2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
Synthetic peptide within Human Desmoglein 2/DSG2 aa 925-975. The exact sequence is proprietary. (NP_001934.2).
Database link: Q14126
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
This product was previously labelled as Desmoglein 2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226184 was affinity purified using an epitope specific to Desmoglein 2/DSG2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 122 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionComponent of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
Tissue specificityAll of the tissues tested and carcinomas.
Involvement in diseaseDefects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10) [MIM:610193]; also known as arrhythmogenic right ventricular cardiomyopathy 10 (ARVC10). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
Genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB) [MIM:612877]. A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similaritiesContains 4 cadherin domains.
DomainCalcium may be bound by the cadherin-like repeats.
Cellular localizationCell membrane. Cell junction > desmosome.
- Information by UniProt
- ARVC 10 antibody
- ARVC10 antibody
- ARVD 10 antibody
All lanes : Anti-Desmoglein 2/DSG2 antibody (ab226184) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 122 kDa
Exposure time: 3 seconds
Desmoglein 2/DSG2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226184 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226184 at 1 µg/ml.
Lane 1: ab226184 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 1 second.
ab226184 has not yet been referenced specifically in any publications.