Overview

  • Product name

    Anti-Desmoglein 2/DSG2 antibody
    See all Desmoglein 2/DSG2 primary antibodies
  • Description

    Rabbit polyclonal to Desmoglein 2/DSG2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide within Human Desmoglein 2/DSG2 aa 925-975. The exact sequence is proprietary. (NP_001934.2).
    Database link: Q14126

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
  • General notes

     This product was previously labelled as Desmoglein 2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab226184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 122 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
  • Tissue specificity

    All of the tissues tested and carcinomas.
  • Involvement in disease

    Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10) [MIM:610193]; also known as arrhythmogenic right ventricular cardiomyopathy 10 (ARVC10). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
    Genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB) [MIM:612877]. A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similarities

    Contains 4 cadherin domains.
  • Domain

    Calcium may be bound by the cadherin-like repeats.
  • Cellular localization

    Cell membrane. Cell junction > desmosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARVC 10 antibody
    • ARVC10 antibody
    • ARVD 10 antibody
    • ARVD10 antibody
    • Cadherin family member 5 antibody
    • CDHF 5 antibody
    • CDHF5 antibody
    • CMD1BB antibody
    • Desmoglein 2 antibody
    • Desmoglein-2 antibody
    • Desmoglein2 antibody
    • DSG 2 antibody
    • DSG2 antibody
    • DSG2_HUMAN antibody
    • HDGC antibody
    • HDGC included antibody
    • Human Desmoglein colon antibody
    • MGC117034 antibody
    • MGC117036 antibody
    • MGC117037 antibody
    see all

Images

  • All lanes : Anti-Desmoglein 2/DSG2 antibody (ab226184) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 122 kDa


    Exposure time: 3 seconds
  • Desmoglein 2/DSG2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226184 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226184 at 1 µg/ml.

    Lane 1: ab226184 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 1 second.

References

ab226184 has not yet been referenced specifically in any publications.

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