Recombinant Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)
![Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)](https://www.abcam.com/ps/products/271/ab271913/Images/ab271913-414434-anti-desmoglein-2dsg2-antibody-epr6768-bsa-and-azide-free-western-blot-hap1-scraped-control-hap1-scraped-hap1-dsg2-knockout-scraped-control-hap1-dsg2-knockout-scraped.jpg "Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6768] to Desmoglein 2/DSG2 - BSA and Azide free
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free
See all Desmoglein 2/DSG2 primary antibodies -
Description
Rabbit monoclonal [EPR6768] to Desmoglein 2/DSG2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Monkey
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HAP1, HepG2, HeLa, Human fetal heart, mouse heart and rat heart lysates. IHC-P: Human thyroid gland papillary carcinoma tissue.
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General notes
ab271913 is the carrier-free version of ab150372.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6768 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 122 kDa.
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| IHC-P |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 122 kDa. |
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IHC-P
Use at an assay dependent concentration. |
Target
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Function
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. -
Tissue specificity
All of the tissues tested and carcinomas. -
Involvement in disease
Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10) [MIM:610193]; also known as arrhythmogenic right ventricular cardiomyopathy 10 (ARVC10). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
Genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB) [MIM:612877]. A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
Sequence similarities
Contains 4 cadherin domains. -
Domain
Calcium may be bound by the cadherin-like repeats. -
Cellular localization
Cell membrane. Cell junction > desmosome. - Information by UniProt
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Database links
- Entrez Gene: 1829 Human
- Entrez Gene: 13511 Mouse
- Entrez Gene: 307562 Rat
- Omim: 125671 Human
- SwissProt: Q14126 Human
- SwissProt: O55111 Mouse
- Unigene: 412597 Human
- Unigene: 345891 Mouse
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Alternative names
- ARVC 10 antibody
- ARVC10 antibody
- ARVD 10 antibody
see all
Images
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Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)All lanes : Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372) at 1/10000 dilution
Lane 1 : Trypsinised HAP1 cell lysate
Lane 2 : Scraped HAP1 cell lysate
Lane 3 : Trypsinised DSG2 knockout HAP1 cell lysate
Lane 4 : Scraped DSG2 knockout HAP1 cell lysate
Lane 5 : Trypsinised A431 cell lysate
Lane 6 : Scraped A431 cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 122 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab150372).
Lanes 1 - 6: Merged signal (red and green). Green - ab150372 observed at 95 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab150372 was shown to react with Desmoglein 2 in wild-type HAP1 cells in western blot with loss of signal observed in DSG2 knockout sample. Wild-type and DSG2 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab150372 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)](https://www.abcam.com/ps/products/271/ab271913/Images/ab271913-1-ab150372156952antidesmoglein2antibodyepr6768immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)Immunohistochemical analysis of paraffin embedded Human thyroid gland papillary carcinoma tissue labeling Desmoglein 2/DSG2 with ab150372 at a 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150372). -
![Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)](https://www.abcam.com/ps/products/271/ab271913/Images/ab271913-3-benefits-of-recombinant-antibodies.png)
Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab271913 has not yet been referenced specifically in any publications.
