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    desmoglein-2dsg2-antibody-epr6768-bsa-and-azide-free-ab271913.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)
  • Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6768] to Desmoglein 2/DSG2 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Unconjugated

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Primary
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Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372)
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Anti-TRAF2 antibody [EPR6048] - BSA and Azide free (ab230795)
Conjugation
Product image
Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)

View more associated products

Overview

  • Product name

    Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free
    See all Desmoglein 2/DSG2 primary antibodies
  • Description

    Rabbit monoclonal [EPR6768] to Desmoglein 2/DSG2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Monkey
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, HepG2, HeLa, Human fetal heart, mouse heart and rat heart lysates. IHC-P: Human thyroid gland papillary carcinoma tissue.
  • General notes

    ab271913 is the carrier-free version of ab150372.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6768
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Cadherins
    • Cardiovascular
    • Heart
    • Cardiac arrhythmias

Associated products

  • Alternative Versions

    • Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • HeLa membrane extract lysate (ab29547)
    • Mouse heart normal tissue lysate - total protein (ab30291)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab271913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 122 kDa.
IHC-P
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 122 kDa.
IHC-P
Use at an assay dependent concentration.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
  • Tissue specificity

    All of the tissues tested and carcinomas.
  • Involvement in disease

    Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10) [MIM:610193]; also known as arrhythmogenic right ventricular cardiomyopathy 10 (ARVC10). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
    Genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB) [MIM:612877]. A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similarities

    Contains 4 cadherin domains.
  • Domain

    Calcium may be bound by the cadherin-like repeats.
  • Cellular localization

    Cell membrane. Cell junction > desmosome.
  • Target information above from: UniProt accession Q14126 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1829 Human
    • Entrez Gene: 13511 Mouse
    • Entrez Gene: 307562 Rat
    • Omim: 125671 Human
    • SwissProt: Q14126 Human
    • SwissProt: O55111 Mouse
    • Unigene: 412597 Human
    • Unigene: 345891 Mouse
    • Alternative names

      • ARVC 10 antibody
      • ARVC10 antibody
      • ARVD 10 antibody
      • ARVD10 antibody
      • Cadherin family member 5 antibody
      • CDHF 5 antibody
      • CDHF5 antibody
      • CMD1BB antibody
      • Desmoglein 2 antibody
      • Desmoglein-2 antibody
      • Desmoglein2 antibody
      • DSG 2 antibody
      • DSG2 antibody
      • DSG2_HUMAN antibody
      • HDGC antibody
      • HDGC included antibody
      • Human Desmoglein colon antibody
      • MGC117034 antibody
      • MGC117036 antibody
      • MGC117037 antibody
      see all

    Images

    • Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)
      Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)
      All lanes : Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372) at 1/10000 dilution

      Lane 1 : Trypsinised HAP1 cell lysate
      Lane 2 : Scraped HAP1 cell lysate
      Lane 3 : Trypsinised DSG2 knockout HAP1 cell lysate
      Lane 4 : Scraped DSG2 knockout HAP1 cell lysate
      Lane 5 : Trypsinised A431 cell lysate
      Lane 6 : Scraped A431 cell lysate

      Lysates/proteins at 40 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 122 kDa
      Observed band size: 95 kDa why is the actual band size different from the predicted?



      This data was developed using the same antibody clone in a different buffer formulation (ab150372).

      Lanes 1 - 6: Merged signal (red and green). Green - ab150372 observed at 95 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

      ab150372 was shown to react with Desmoglein 2 in wild-type HAP1 cells in western blot with loss of signal observed in DSG2 knockout sample. Wild-type and DSG2 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab150372 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)

      Immunohistochemical analysis of paraffin embedded Human thyroid gland papillary carcinoma tissue labeling Desmoglein 2/DSG2 with ab150372 at a 1/250 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150372).
    • Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)
      Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab271913? Please let us know so that we can cite the reference in this datasheet.

    ab271913 has not yet been referenced specifically in any publications.

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