Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Desmoglein 2/DSG2 antibody [EPR6768] - Low endotoxin, Azide free (ab230833)

Overview

  • Product name

    Anti-Desmoglein 2/DSG2 antibody [EPR6768] - Low endotoxin, Azide free
    See all Desmoglein 2/DSG2 primary antibodies
  • Description

    Rabbit monoclonal [EPR6768] to Desmoglein 2/DSG2 - Low endotoxin, Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    aa 1100-1200 (C terminal).

  • Positive control

    • HepG2, HeLa, Human fetal heart, mouse heart and rat heart lysates; Human thyroid gland papillary carcinoma tissue; HepG2 cells
  • General notes

    ab230833 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    This product was previously labelled as Desmoglein 2

Properties

Applications

Our Abpromise guarantee covers the use of ab230833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 122 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
    • Tissue specificity

      All of the tissues tested and carcinomas.
    • Involvement in disease

      Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10) [MIM:610193]; also known as arrhythmogenic right ventricular cardiomyopathy 10 (ARVC10). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
      Genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB) [MIM:612877]. A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    • Sequence similarities

      Contains 4 cadherin domains.
    • Domain

      Calcium may be bound by the cadherin-like repeats.
    • Cellular localization

      Cell membrane. Cell junction > desmosome.
    • Information by UniProt
    • Database links

    • Alternative names

      • ARVC 10 antibody
      • ARVC10 antibody
      • ARVD 10 antibody
      • ARVD10 antibody
      • Cadherin family member 5 antibody
      • CDHF 5 antibody
      • CDHF5 antibody
      • CMD1BB antibody
      • Desmoglein 2 antibody
      • Desmoglein-2 antibody
      • Desmoglein2 antibody
      • DSG 2 antibody
      • DSG2 antibody
      • DSG2_HUMAN antibody
      • HDGC antibody
      • HDGC included antibody
      • Human Desmoglein colon antibody
      • MGC117034 antibody
      • MGC117036 antibody
      • MGC117037 antibody
      see all

    Images

    • This WB data was generated using the same anti-Desmoglein 2/DSG2 antibody clone, EPR6768, in a different buffer formulation (cat# ab150372).

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Desmoglein 2/DSG2 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: A431 whole cell lysate (20 µg)
      Lane 4: A549 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab150372 observed at 90-160  kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab150372 was shown to specifically react with Desmoglein 2/DSG2 in wild-type HAP1 cells as signal was lost in Desmoglein 2/DSG2 knockout cells. Wild-type and Desmoglein 2/DSG2 knockout samples were subjected to SDS-PAGE. Ab150372 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • This IHC data was generated using the same anti-Desmoglein 2/DSG2 antibody clone, EPR6768, in a different buffer formulation (cat# ab150372).

      Immunohistochemical analysis of paraffin embedded Human thyroid gland papillary carcinoma tissue labeling Desmoglein 2/DSG2 with ab150372 at a 1/250 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab230833 has not yet been referenced specifically in any publications.

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