Overview

  • Product name
    Anti-Desmoglein 3 / PVA antibody [5G11]
    See all Desmoglein 3 / PVA primary antibodies
  • Description
    Mouse monoclonal [5G11] to Desmoglein 3 / PVA
  • Host species
    Mouse
  • Specificity
    Reacts specifically with human Desmoglein 3 / PVA protein. Does not crossreact with Desmoglein 1 or Desmoglein 2.
  • Tested applications
    Suitable for: IP, ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Desmoglein 3/ PVA.

  • Positive control
    • IHC-P: FFPE human skin melanoma tissue sections.
  • General notes

    This antibody clone is manufactured by Abcam.

    The clone number has been updated from 3G133 to 5G11 since both name the same clone and 5G11 is the original clone number.

    For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Previously labelled as Desmoglein 3. 

Properties

Applications

Our Abpromise guarantee covers the use of ab14416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 119.9 kDa.

Target

  • Function
    Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
  • Tissue specificity
    Epidermis, tongue, tonsil, esophagus and carcinomas.
  • Sequence similarities
    Contains 4 cadherin domains.
  • Domain
    Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.
  • Cellular localization
    Cell membrane. Cell junction > desmosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • 130 kD pemphigus vulgaris antigen antibody
    • 130 kDa pemphigus vulgaris antigen antibody
    • Balding antibody
    • Cadherin family member 6 antibody
    • CDHF 6 antibody
    • CDHF6 antibody
    • Desmoglein 3 antibody
    • Desmoglein 3 precursor antibody
    • Desmoglein-3 antibody
    • Desmoglein3 antibody
    • DKFZp686P23184 antibody
    • DSG 3 antibody
    • DSG3 antibody
    • DSG3_HUMAN antibody
    • Pemphigus vulgaris antigen antibody
    • PVA antibody
    see all

Images

  • IHC image of Desmoglein 3 / PVA staining in Human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14416, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemical analysis of Human tongue tissue, staining Desmoglein 3 / PVA with ab14416.

    Tissue was fixed with formaldehyde and antigen retrieval was by proteinase K. Samples were incubated with primary antibody (1/500 in Tris buffered saline with 0.1% Tween 20) for 18 hours at 4°C. An HRP-conjugated goat anti-mouse polyclonal IgG (1/2) was used as the secondary antibody.

    See Abreview

References

ab14416 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (skin)
Permeabilization
No
Specification
skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted May 25 2015

Answer

Gracias por contactarnos.

En un vial conteniendo aproximadamente 100ul de anticuerpo están contenidos 50ug de inmunoglobulina a una concentración de 0.5 mg/ml.

Espero que esta información os aclare la pregunta. En caso contrario no dudéis en contactarnos de nuevo.

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Question
Answer

Thank you for contacting us.

xxxxxxxxx is currently out of the office so I have taken over this case.

With regards to your question, a clone number is given to an antibody produced by a single clone of hybridoma cells. Each hybridoma cell clone produces one single pure homogeneous type of antibody. The term "monoclonal" pertains to a single clone of cells, a single cell and the progeny of that cell. Monoclonal antibodies can be made in large amounts in the laboratory.

Each clone number represents a specific cell line cloned from ascites that was used to manufacture the antibody. Since antibodies are produced by more than one host, each cloned cell line receives a unique clone number. The clone number is not synonymous with the lot number which is in relation with the creation of the vial. There is little to no difference amongst the quality of the clones.

Therefore, with regards to your question, there are no clones that are commonly used for Desmoglein 3 for lung SCC. A clone is just a number given to the hybridoma from which the antibodies are produced from.

Other than ab125710 which failed to give positive results, I can suggest Anti-Desmoglein 3 antibody [3G133] (ab14416) (https://www.abcam.com/ab14416) or Anti-Desmoglein 3 antibody [MM0245- 4W25] (ab89177) (https://www.abcam.com/ab89177)

These two antibodies are produced from different clones: 3G133 or MM0245- 4W25. Neither of these has an advantage for detecting Desmoglein 3 in lung SCC both should work on human tissues. The difference between these two antibodies is the immunogen. ab14416's immunogen is Recombinant fragment (Human Desmoglein 3 extracellular domain) whereas ab89177's immunogen is Recombinant full length human Desmoglein 3 protein.

Please note that these antibodies are guaranteed to function in immunhistochemistry in human but we have no specific results for use on lung SCC.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tongue)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: proteinase K
Permeabilization
No
Specification
Tongue
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 20 2012

Question
Answer

DISCOUNT CODE: *** Expiration date: February 7th, 2011 I am very pleased to hear you would like to accept our offer and test ab14416 in mouse. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for your enquiry. There is a very helpful protocol on our website for IHC in paraffin embedded sections that will answer your questions about how to prepare the tissue sections. The protocol can be found by clicking on the Resources tab on the home page, then protocols, then Immunhohistochemistry, and then for paraffin embedded sections. This protein is found in epidermis, tongue, tonsil, esophagus and carcinomas, so any of those tissues would be good positive controls. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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