Overview

  • Product name

    Anti-Desmoglein 3/PVA antibody [5H10]
    See all Desmoglein 3/PVA primary antibodies
  • Description

    Mouse monoclonal [5H10] to Desmoglein 3/PVA
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Fusion protein corresponding to Human Desmoglein 3/PVA. (Recombinant maltose binding protein fused to the extracellular domain of human Desmoglein3).
    Database link: P32926

  • Positive control

    • ICC/IF: A431 cells IHC-Fr: Human skin
  • General notes

    This antibody clone is manufactured by Abcam. If you require it in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab231309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.

This product gave a positive signal in cells fixed with 4% formaldehyde (10 minutes) and 100% methanol (5 minutes).

IHC-Fr Use a concentration of 1 µg/ml.

Tissue sections were fixed with 10% PFA

Target

  • Function

    Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
  • Tissue specificity

    Epidermis, tongue, tonsil, esophagus and carcinomas.
  • Sequence similarities

    Contains 4 cadherin domains.
  • Domain

    Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.
  • Cellular localization

    Cell membrane. Cell junction > desmosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • 130 kD pemphigus vulgaris antigen antibody
    • 130 kDa pemphigus vulgaris antigen antibody
    • Balding antibody
    • Cadherin family member 6 antibody
    • CDHF 6 antibody
    • CDHF6 antibody
    • Desmoglein 3 antibody
    • Desmoglein 3 precursor antibody
    • Desmoglein-3 antibody
    • Desmoglein3 antibody
    • DKFZp686P23184 antibody
    • DSG 3 antibody
    • DSG3 antibody
    • DSG3_HUMAN antibody
    • Pemphigus vulgaris antigen antibody
    • PVA antibody
    see all

Images

  • All lanes : Anti-Desmoglein 3/PVA antibody [5H10] (ab231309) at 5 µg/ml

    Lane 1 : A431 Whole Cell Lysate
    Lane 2 : Human Skin Tissue Lysate

    Lysates/proteins at 40 µg per lane.

    Performed under reducing conditions.


    This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 3% milk before ab231309 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 5ug/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • IHC image of ab231309 staining in 10% formaldehyde fixed frozen tissue section of normal human skin.

    Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with ab231309 (1 μg/ml) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647)) and DAPI for 1 hour at room temperature.

    The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab231309. 

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such antibody concentrations and incubation times.

  • ab231309 staining Desmoglein 3/PVA in A431 (human epidermoid carcinoma cell line) cells.

    The cells were fixed with 100% methanol (5 minutes), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated overnight at +4°C with ab231309 at 1 μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red).

    Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

This product has been referenced in:

  • Mahoney MG  et al. Delineation of diversified desmoglein distribution in stratified squamous epithelia: implications in diseases. Exp Dermatol 15:101-9 (2006). Read more (PubMed: 16433681) »
  • Wahl JK Generation of monoclonal antibodies specific for desmoglein family members. Hybrid Hybridomics 21:37-44 (2002). Human . Read more (PubMed: 11991815) »
See all 2 Publications for this product

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