Key features and details
- Mouse monoclonal [5H10] to Desmoglein 3/PVA
- Suitable for: ICC/IF, IHC-Fr, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Desmoglein 3/PVA antibody [5H10]
See all Desmoglein 3/PVA primary antibodies
DescriptionMouse monoclonal [5H10] to Desmoglein 3/PVA
Tested applicationsSuitable for: ICC/IF, IHC-Fr, WBmore details
Species reactivityReacts with: Human
Fusion protein corresponding to Human Desmoglein 3/PVA. (Recombinant maltose binding protein fused to the extracellular domain of human Desmoglein3).
Database link: P32926
- ICC/IF: A431 cells IHC-Fr: Human skin
This antibody clone is manufactured by Abcam. If you require it in a particular buffer formulation or a particular conjugate for your experiments, please contact email@example.com.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS.
Light chain typekappa
Our Abpromise guarantee covers the use of ab231309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.
This product gave a positive signal in cells fixed with 4% formaldehyde (10 minutes) and 100% methanol (5 minutes).
|IHC-Fr||Use a concentration of 1 µg/ml.
Tissue sections were fixed with 10% PFA
|WB||Use a concentration of 5 µg/ml. Predicted molecular weight: 108 kDa.|
FunctionComponent of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
Tissue specificityEpidermis, tongue, tonsil, esophagus and carcinomas.
Sequence similaritiesContains 4 cadherin domains.
DomainThree calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.
Cellular localizationCell membrane. Cell junction > desmosome.
- Information by UniProt
- 130 kD pemphigus vulgaris antigen antibody
- 130 kDa pemphigus vulgaris antigen antibody
- Balding antibody
All lanes : Anti-Desmoglein 3/PVA antibody [5H10] (ab231309) at 5 µg/ml
Lane 1 : A431 Whole Cell Lysate
Lane 2 : Human Skin Tissue Lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 108 kDa
This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 3% milk before ab231309 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 5ug/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
IHC image of ab231309 staining in 10% formaldehyde fixed frozen tissue section of normal human skin.
Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with ab231309 (1 μg/ml) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647)) and DAPI for 1 hour at room temperature.
The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab231309.
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such antibody concentrations and incubation times.
ab231309 staining Desmoglein 3/PVA in A431 (human epidermoid carcinoma cell line) cells.
The cells were fixed with 100% methanol (5 minutes), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated overnight at +4°C with ab231309 at 1 μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red).
Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231309 has been referenced in 2 publications.
- Mahoney MG et al. Delineation of diversified desmoglein distribution in stratified squamous epithelia: implications in diseases. Exp Dermatol 15:101-9 (2006). PubMed: 16433681
- Wahl JK Generation of monoclonal antibodies specific for desmoglein family members. Hybrid Hybridomics 21:37-44 (2002). Human . PubMed: 11991815