Key features and details
- Rabbit polyclonal to Desmoglein 3/PVA
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-Desmoglein 3/PVA antibody
See all Desmoglein 3/PVA primary antibodies
DescriptionRabbit polyclonal to Desmoglein 3/PVA
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human, Pig
Recombinant fragment corresponding to Human Desmoglein 3/PVA aa 858-999. N-terminal His-tag and GST-tags (Expressed in E.coli).
EISLGVDGEGKEVQPPSKDSGYGIESCGHPIEVQQTGFVKCQTLSGSQGA SALSTSGSVQPAVSIPDPLQHGNYLVTETYSASGSLVQPSTAGFDPLLTQ NVIVTERVICPISSVPGNLAGPTQLRGSHTMLCTEDPCSRLI
Database link: P32926
- Pig skin tissue; Recombinant human Desmoglein 3/PVA protein.
Previously labelled as Desmoglein 3.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml.|
FunctionComponent of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
Tissue specificityEpidermis, tongue, tonsil, esophagus and carcinomas.
Sequence similaritiesContains 4 cadherin domains.
DomainThree calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.
Cellular localizationCell membrane. Cell junction > desmosome.
- Information by UniProt
- 130 kD pemphigus vulgaris antigen antibody
- 130 kDa pemphigus vulgaris antigen antibody
- Balding antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human placenta tissues staining Desmoglein 3/PVA antibody at ab231947 20 µg/mL
Anti-Desmoglein 3/PVA antibody (ab231947) at 2 µg/ml + Recombinant human Desmoglein 3/PVA protein.
Anti-Desmoglein 3/PVA antibody (ab231947) at 3 µg/ml + Pig skin tissue lysate.
HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231947 has not yet been referenced specifically in any publications.