Key features and details
- Rabbit polyclonal to Desmoglein 3/PVA - N-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Desmoglein 3/PVA antibody - N-terminal
See all Desmoglein 3/PVA primary antibodies
DescriptionRabbit polyclonal to Desmoglein 3/PVA - N-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Desmoglein 3/PVA (N terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: P32926
- WB: A431 whole cell extract. IP: A431 whole cell extract.
Previously labelled as Desmoglein 3.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 108 kDa.|
|IP||1/100 - 1/500.|
FunctionComponent of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
Tissue specificityEpidermis, tongue, tonsil, esophagus and carcinomas.
Sequence similaritiesContains 4 cadherin domains.
DomainThree calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.
Cellular localizationCell membrane. Cell junction > desmosome.
- Information by UniProt
- 130 kD pemphigus vulgaris antigen antibody
- 130 kDa pemphigus vulgaris antigen antibody
- Balding antibody
Anti-Desmoglein 3/PVA antibody - N-terminal (ab228726) at 1/1000 dilution + A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg
Predicted band size: 108 kDa
7.5% SDS-PAGE gel.
Desmoglein 3/PVA was immunoprecipitated from A431 (human epidermoid carcinoma cell line) whole cell extract with 5 µg ab228726. Western blot was performed from the immunoprecipitate using ab228726. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: A431 whole cell extract.
Lane 2: Control IgG IP in A431 whole cell extract.
Lane 3: ab228726 IP in A431 whole cell extract.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228726 has not yet been referenced specifically in any publications.