Anti-Desmoglein 4 antibody (ab230787)
Key features and details
- Rabbit polyclonal to Desmoglein 4
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Desmoglein 4 antibody
See all Desmoglein 4 primary antibodies -
Description
Rabbit polyclonal to Desmoglein 4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Desmoglein 4 (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: Q86SJ6 -
Positive control
- WB: HEK-293T, SP2/0 and H9c2 whole cell lysates. IHC-P: Human prostate and lung respiratory epithelium tissues.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab230787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | 1/500 - 1/1000. |
Target
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Relevance
DSG4(Desmoglein 4) is a component of intercellular desmosome junctions. DSG4 is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. DSG4 coordinates the transition from proliferation to differentiation in hair follicle keratinocytes. The essential role of desmoglein 4 in skin was established by identifying mutations in families with inherited hypotrichosis, as well as in the lanceolate hair mouse. The human desmoglein 4 gene (DSG4) demonstrates that it is composed of 16 exons spanning approximately 37 kb of 18q12 and is situated between DSG1 and DSG3. Defects in DSG4 are the cause of localized autosomal hypotrichosis (LAH). LAH is an autosomal recessive skin disorder. DSG4 is one of the target molecules recognized by autoantibodies in patients with pemphigus vulgaris. Pemphigus vulgaris is a potentially lethal skin disease in which epidermal blisters occur as the result of the loss of cell-cell adhesion. -
Cellular localization
Cell membrane; single pass type I membrane protein. -
Database links
- Entrez Gene: 147409 Human
- Entrez Gene: 16769 Mouse
- Entrez Gene: 291754 Rat
- Omim: 607892 Human
- SwissProt: Q86SJ6 Human
- SwissProt: Q7TMD7 Mouse
- SwissProt: Q6W3B0 Rat
- Unigene: 407618 Human
see all -
Alternative names
- CDGF 13 antibody
- CDGF13 antibody
- CDH family member 13 antibody
see all
Images
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Western blot - Anti-Desmoglein 4 antibody (ab230787)All lanes : Anti-Desmoglein 4 antibody (ab230787) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : SP2/0 whole cell lysate
Lane 3 : H9c2 (rat heart myoblast cell line) whole cell lysate
Developed using the ECL technique. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 4 antibody (ab230787)Formalin-fixed, paraffin-embedded human prostate tissue stained for Desmoglein 4 with ab230787 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 4 antibody (ab230787)Formalin-fixed, paraffin-embedded human lung respiratory epithelium tissue stained for Desmoglein 4 with ab230787 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab230787 has not yet been referenced specifically in any publications.
