Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)
- Datasheet
- Specific References (4)
- Protocols
Overview
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Product nameAnti-Desmoplakin antibody [EPR4383(2)]
See all Desmoplakin primary antibodies -
DescriptionRabbit monoclonal [EPR4383(2)] to Desmoplakin
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Host speciesRabbit
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Tested applicationsSuitable for: Flow Cyt, WB, ICC/IFmore details
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Species reactivityReacts with: Rat, Human
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Immunogen
Synthetic peptide within Human Desmoplakin aa 400-500. The exact sequence is proprietary.
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Positive control
- WB: A431, HACAT and BXPC-3 cell lysates. ICC/IF: A431 cells. Flow Cyt: A431 cells.
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR4383(2)
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab109445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/250. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 332 kDa. | |
| ICC/IF | 1/1000. |
Target
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RelevanceDesmosomes are the most common type of intercellular junction in vertebrate epithelial cells. They are characterized by 2 forms of interaction with other cellular structures. First, they form membrane anchorage sites for intermediate-size filaments, which are seen as electron-dense plaques evident beneath the plasma membrane. Second, a specific membrane core domain interacts with a corresponding domain of the plasma membrane of an adjacent cell, apparently mediating intercellular adhesion in a stable way. The desmosome intermediate filament complex is thought to impart tensile strength and resilience to the epithelium. Desmosomal proteins can be divided into 2 groups based on whether they fractionate with the urea-insoluble 'core' or the urea-soluble 'plaque' components. Desmoglein is, for example, a protein of the core. The main proteins of the plaque comprise the desmoplakins and plakoglobin.
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Cellular localizationCell junction, desmosome. Cytoplasm, cytoskeleton.
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Database links
- Entrez Gene: 1832 Human
- Entrez Gene: 306871 Rat
- Omim: 125647 Human
- SwissProt: P15924 Human
- Unigene: 519873 Human
- Unigene: 231913 Rat
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Alternative names
- 250/210 kDa paraneoplastic pemphigus antigen antibody
- Desmoplakin I antibody
- Desmoplakin II antibody
see all
Images
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![Western blot - Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)](https://www.abcam.com/ps/products/109/ab109445/Images/Desmoplakin-Primary-antibodies-ab109445-1.jpg)
Western blot - Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)All lanes : Anti-Desmoplakin antibody [EPR4383(2)] (ab109445) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HACAT cell lysate
Lane 3 : BXPC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 332 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)](https://www.abcam.com/ps/products/109/ab109445/Images/ab109445-261200-ab109445-A431-methanol-1000.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)Immunocytochemistry/Immunofluorescence analysis of A431 cells labelling Desmoplakin with ab109445 at 1/1000. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI. -
![Flow Cytometry - Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)](https://www.abcam.com/ps/products/109/ab109445/Images/ab109445-287273-ab109445FC1.jpg)
Flow Cytometry - Anti-Desmoplakin antibody [EPR4383(2)] (ab109445)Flow cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling Desmoplakin (red) with ab109445 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
References
This product has been referenced in:
- Ortega A et al. Intercalated disc in failing hearts from patients with dilated cardiomyopathy: Its role in the depressed left ventricular function. PLoS One 12:e0185062 (2017). Read more (PubMed: 28934278) »
- Hartlieb E et al. Desmoglein 2 Compensates for Desmoglein 3 but Does Not Control Cell Adhesion via Regulation of p38 Mitogen-activated Protein Kinase in Keratinocytes. J Biol Chem 289:17043-53 (2014). WB . Read more (PubMed: 24782306) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"