Product nameAnti-Desmoplakin antibody [EPR4383(2)]
See all Desmoplakin primary antibodies
DescriptionRabbit monoclonal [EPR4383(2)] to Desmoplakin
Tested applicationsSuitable for: Flow Cyt, WB, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Synthetic peptide within Human Desmoplakin aa 400-500. The exact sequence is proprietary.
Database link: P15924
- WB: A431, HACAT and BXPC-3 cell lysates. ICC/IF: A431 cells. Flow Cyt: A431 cells.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 332 kDa.|
RelevanceDesmosomes are the most common type of intercellular junction in vertebrate epithelial cells. They are characterized by 2 forms of interaction with other cellular structures. First, they form membrane anchorage sites for intermediate-size filaments, which are seen as electron-dense plaques evident beneath the plasma membrane. Second, a specific membrane core domain interacts with a corresponding domain of the plasma membrane of an adjacent cell, apparently mediating intercellular adhesion in a stable way. The desmosome intermediate filament complex is thought to impart tensile strength and resilience to the epithelium. Desmosomal proteins can be divided into 2 groups based on whether they fractionate with the urea-insoluble 'core' or the urea-soluble 'plaque' components. Desmoglein is, for example, a protein of the core. The main proteins of the plaque comprise the desmoplakins and plakoglobin.
Cellular localizationCell junction, desmosome. Cytoplasm, cytoskeleton.
- 250/210 kDa paraneoplastic pemphigus antigen antibody
- Desmoplakin I antibody
- Desmoplakin II antibody
All lanes : Anti-Desmoplakin antibody [EPR4383(2)] (ab109445) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HACAT cell lysate
Lane 3 : BXPC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 332 kDa
Immunocytochemistry/Immunofluorescence analysis of A431 cells labelling Desmoplakin with ab109445 at 1/1000. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI.
Flow cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling Desmoplakin (red) with ab109445 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
This product has been referenced in:
- Zhang R et al. Mechanical strain triggers differentiation of dental mesenchymal stem cells by activating osteogenesis-specific biomarkers expression. Am J Transl Res 11:233-244 (2019). Read more (PubMed: 30787982) »
- Ng R et al. Patient mutations linked to arrhythmogenic cardiomyopathy enhance calpain-mediated desmoplakin degradation. JCI Insight 5:N/A (2019). Read more (PubMed: 31194698) »